>Homo sapiens RNF225
MPCPRPFWLRHSRAPQGSGPSSPGSLSAPRSPSRGEDQEEEEEEEGDGSPGSGPILPPAS
PVECLICVSSFDGVFKLPKRLDCGHVFCLECLARLSLATAGGGNAVACPVCRAPTRLAPR
RGLPALPTQSGLLPRDARAPPSRQGSVRFDRRRGLLYLRPPPPPPGPRKARAPPPPPPLR
LGRPLSRRLSLASPAWVFNAAVALAVLVAAGLVVSGVYIFFLIPHATSSGPPRPQLVALA
PAPGFSWFPPRPPPGSPWAPAWTPRPTGPDLDTALPGTAEDALEPEAGPEDPAEAERTLD
RRSDGTWGTEAGPGWAPWPRGARRLWGSQ

>Gorilla gorilla RNF225
MPCPRPFWLRHSRAPQGSGPSSPGSLSAPRSPSRGEDQEEEEEEEGDGSPGSGPILPPAS
PVECLICVSSFDGVFKLPKRLDCGHVFCLECLARLSLATAGGGNAVACPPPPPGPRKARA
PPPPPPLRLGRPLSRRLSLASPAWVFNAAVALAVLVAAGLVVSGVYIFFLIPHATSSGPA
RPQLVALAPAPGFSWFPPRPPPGSPWTPAWTPRATGPDLD

>Pongo abelii RNF225
MPCPRPFWLRHFRAPQGSGPSSPGSLSAPCSPSRGEDQEEEEGDGSPGSGPILSPASPVE
CLICVSSFDGVFKLPKRLDCGHVFCLECLARLSLATAGGGNAVACPVCRAPTRLAPRRGL
PALPTQSGLLPRDARAPPSRQGSVRFDRRRGLLYLRPPPPPPGPRKARAPPPPPPLRLGR
PLSRRLSLASPAWVFNAAVALAVLVAAGLVVSGVYIFFLIPHATSSGPARPQLVALAPAP
GFSWFPPRPPPGSPWTPAWTPRPTGPDLDTALPGTAEDALEPEAGPEDPAEAEGTLDRRS
DGTWGTEAGPGWAP

>Macaca mulatta RNF225
MPCPRPFWLRHSRAPQGSGPSPPSRDGSPGSSPILSPASPVECLICVSSFDGVFKLPKRL
DCGHVFCLECLARLSLATAGGGNAVACPVCRAPTRLAPRRGLPALPTQSGLLPRDARAPP
SRQGSVRFVSLASPAWVFNAAVALAVLVAAGLVVSGVYIFFLIPHATSSGPARPQLVALA
PAPGFSWFPPRPTPGVPWTPAWTPRPTGPDLDTTLPGTAEDALEPQAGPEDPVENEGTLD
RPSDGTWGTEADPGWAP

>Callithrix jacchus RNF225
MPCPRPPWLRRSRALQGSGPSSPGSLSAPRSPSRGEDQDQDEEEEGDCSPGSDPVLLPAS
PVECLICVSPFDGVFKLPKRLDCGHVFCLECLARLSLATAGGGDAVACPVCRAPTRLAPR
RGLPALPTQSALLPRDARAPPSRQGSVRFDRRRGLLYLRPPPPPPGPRKSRAPRAQPPPP
PLRLGRPLSRRLTLDSPAWVFNAAVAVAVLVTAGLVVSGVYIFFLIPHATSSGPARPQLV
ALAPAPGFSWFPPRPTPGAPWTPAWTPRPTGPDLD

>Mus musculus RNF225
MPCPRLPWLRRHRTSQGSGPSSPSTVSAPNSPSRGEDEDAEEEEGDGTPGSGPILPPTSP
MECLICVSPFDGIFKLPKRLDCGHVFCLECLARLSLATAGGGDAVACPMCRAPTRLAPRR
GLPALPTQPGLLPRDARAPLPRQGSVRFDRRRGLLYLRPPPPSPGPRKSRTVRAPPPPPP
LRLGRPLSRRLSLSSPAWAFNAAVALAVLVAAGLVVSGVYIFFLIPHVTNSGVRPQTVAL
APENDFWVSPRPTPVAPWTHAWTRRPTKPDLDLDDTLPEATKDTPELEEATKDPVETQGI
PDLPPDQTPKAEIDLNWNPKAQADGKKVQLQQ

>Rattus norvegicus RNF225
MPCPRLPWLRRHRTSQGSSPGSPSTLSAPNFPSRGEDEDAEEEEGDGTPGSGPILPPASP
MECLICVSPFDGIFKLPKRLDCGHVFCLECLARLSLATAGGGDAVACPMCRAPTRLAPRR
GLPALPTQPGLLPCDARAPLPRQGSVRFDRRRGLLYLRPPPPSPGPRKSRTVRAPPPPPP
LRLGRPLSRRLSLSSPAWAFNAAVALAVLVAAGLVVSGVYIFFLIPHVTTSSPARPQIVA
LAPENGFWVSPRPTPVAPWTHTWTRRPTKPDLDDTLTETTKDTPELEATKGPVEIQETPD
LSPDQTPNAETDLSWDRKAWTDGKRVQLQQ

>Canis familiaris RNF225
MPCPRPPWLPRPRTPQGSSPSSPSSLSATRSPSREGGEDDDGEEEEGDSSPVLGPILPSA
SPVECLICVSPFDGVFKLPKRLDCGHVFCLECLARLSLATAGGGDAVACPVCRAPTRLAP
RRGLPALPTQPGLLPRDAHVAPPRQGSVRFDRRRGLLYLRPPPPPPGPRKFRAARPPPPP
PPLRLGRPLSRRLSWTSPAWAFNAAVALAVLVAAGLVVSGVYIFFLIPHATSSGPARPQL
VALAPASGFSWFPPQPASGTPWTPAWTPRPVGPDQDTALPGAAEDVLEPEGVSEDTAEVE
GTLDRPPD

>Myotis lucifugus RNF225
MSEGESKDSSGSECPVCYEKFRDLEGASRTLSCGHVFCHDCLVKYLLSTRVDGQVQRTIV
CPICRYVTFLSKKSSRWPSMLDKSAQTLAVPRACPPGPPGGQRPGGHTHPTAGSQLVWRA
SPSQSPQLPLDLLPSLPREPQIFIISRHGMPLGEQDSVLPRRSLAELSEASPAPRSTRSF
CCRSRALLLITLIAVVAVVAAILPWVLLVRKQA

>Taeniopygia guttata RNF225
GSPLGSPKPASPVECSICFTSYDNSFKTPKLLQCSHVFCLECVARLSAALAAGQEALPCP
FCRQPTCLPRQGAPGLATSRELLATLPPELQRERQLWMDGTRLCCRRASADAENPDSCDS
IDVAMAKAESAEVAPAGLAGRLSRCDICDDWKRIVLLSALLIILFCIILWPVQCALKTGN
LRCFAR

>Danio rerio RNF225 a
KVSSHPECSICFNTYDNVFKTPKQLDCTHTFCLECLSRIMTTSMDPQNSKIPCPFCRQPT
TIPKKGPPALTTSQEVLCRLPIHQQHEEPVWLDGERLCYQRPIEMPGVPAFCICVDIGAS
GHSGVSSSQTRPRLSLMERMMDWKRLLIFIFLMVLLLGIVLWPLQCIVTTGSLRCSPQNM
HMSTTTASTTTVTMLLLLGDE

>Takifugu rubripes RNF225 b
LKMKCAQTDIEEDCPPPDEIECKICYQHYNAYKRKPKILDCLHRVCTQCLNKILDVAEGA
GFILCPFCRHQTPITDSEVSALPDDANIMSRLAMQEKSWSSDHTGEVLLTPKSFSSSSPS
PDSSSCLVITMTEEQRDSEHAPRQNSAIYPEQSLESVSVGSDGHVDQYATSKLCHHVPRT
LVWLLGFLYFGSLPLGIYLLVLQKVTLGIVSVSLVPTSLTVCLVYEFCSVCDNSVDCMYT
L

>Gasterosteus aculeatus RNF225 b
MSGAQAEIEEKDSPPPEELECKICYQRYNAHHRRPKVLDCLHRVCVRCLIKILDIADGTD
FISCPFCRHQTEVSEYEVSALPDDAIIMSRLAMRDKSWSSDKNREVLLTPTSFSSSSPSR
DSSNCLVITIMEVQGDSQRSPTRDGSSDVFAEQSLDSVSMGSNGLADQDALSKFCNHVPR
ILVWLLGFLYFGSLPLGIYLLVIQRVTLGIACVSLVPSSLTVCLVYGFCQCLCQGMCDCY
S

>Oryzias latipes RNF225 a
MNEGREAGGPPGGNDPPLAPNVKPKPNQNVKNTKEEHRSKKKTVKVKRSRSTDSQRGERG
RRRERERERGSRGRSEDTQRRERRRKPPENDVEDTECSICFCSYDNIFKTPKVLACGHTF
CLECLARINVTSAELKNLPCPVCREVTELRHGRDLPRLDNNQEIIQKLPSQMQRARSIR

>Oryzias latipes RNF225 b
MIGPQLEEVGDKDCPSPEELECKICYQRYNAHNRKPKILDCLHRVCARCLLKILDVADGG
GSIPCPFCRHQTEISEYQVSALPDDVNIMSHLEKSSSSDQNREVLLTPQSFSSSSPSRDS
SNCLVITIMEVQRDSGHSPSQNGSSDIYAEQSLDSVSIGSNGPADHDALTKLCNHVPRIL
VWLLGFLYFGSLPLGIYLLVIQRVTLGIVCVSLVPSSLTVCLVYGFCQCLCQGMCDCSSR
G>Ciona intestinalis RNF183
MAMKYKSSDSRKVPAQPPQWNQFLICSVCENEFNRTDRCPISLGCGHTVCRGCLGDLKHP
QCQFDQNSITCDISDLPVNSALLLLVPEEESHKGSVEMRGVSQKGKENFHPGNIAQCVKL
YDKSKKHIEELALLLRPNKGNELSRPMQRKLVALINCQLVEEEGRKRALRAGRALGERSA
TELILLHQNPTTLSASLWAAVRARGCQFLGPAMQE

>Ciona savignyi RNF183
MENRRTHIEASDVDLALEEAGHCGLYHAWLILLMAVPNILVGAHMGSSVFEAMIPTHYCV
TQQADDITTACNITWNPTNDALAVPEDLDGKHSPCHMYYYDNVTDIDCKFWTLDKNSTPT
VEPGIRTCDHWIFD

>Branchiostoma floridae RNF183
MELQCGLCGQQYSRPRVLSCLHTFCLACLESLMKRQDREGEVCCPVCQQRTLLPSSDPSTLPSDPIIDAL
LEVKSVEAGAVTCTSCEENSSAVCRCLDCAAFLCRNCQGAHRFMKCFKSHRLVELENLSISDLASPLKLL
TCTKHTGEFLEFYCNTCDALVCLKCTAIDHRRTSHQYVYVKDAAAVMKERMLYLLENGRKRSEEVRVQLL
RMEEKIDHVEVEKMKVKGEVDKVFHTLLSKLEDRQEEMCKEVETLYLRQKQFLNKQGKSLQLDEERLQAG
TRFVEKLMTYGSTSDLLTCGKLVTSQFDLLFKQCPGNDMKSSGAVGVSVDYHAFGNLLSRIQIQIKTVPK
IGVSSPVTQAGCSAVGESHRVESGSAEATPPSPKEQFSPSSSRSNLSEERKGSDSAQMDVTELHKVRSFG
KRENAGNCPYLTCVAVGANGNIYALDMYKSVVQVFDKEGKYCFNIGGRFQAPGRLEKPVELATSSKGYIA
VRESNGRMQIYSDTGQFIFKFQLLRVQRPRSMAIDRKGRFLVGDVGTDRCPHGVVIYSSDGKLLRKITKL
SAYTSSLAMSAQNHLLVVDHRAHCIQVYSEEGNHLRTIYPRGSRIYMRGIIVNQNKIILVTSEKVRNNMY
CGGLVVMAKDGTVLDKFKFPEKQPVVHVALTQDGCPLAILQGNSDICLYGSIYSK

>Drosophila melanogaster RNF183
MNNSPKDPYRGGGGGSGPQDGAGSTGVSINIESDDNDSTTAEHDYLLPASSSSVTSGGGV
GGGGVVSGGSTSTSVNLDHQHVPTARSSSLSGGNVRHGNILSTFLARQRSYTPASNSSSP
VRVTTQMNRRLQQSRSMGANTGGSLEEPSSAGAAGGPGASPGTAAGVRQMGFWRVNLNDV
FSLSTASSTEALRSFISHSNFRYPPATTAAPAAPGPQPAANPVDNSHILLGQQQPSMPEP
VSPLRYGRVSAVSGASSAGAMGRSISLREGEMRHNHSLNGGRHNASAIGLNSNPAAFDER
EANESQDLDESDGNVENGGGGGNPPEPAENNPEDEHLISDDMVVQILSHFVRYLPLIFIL
FFKFLHDHLLGIVDLLVLQTVMYNVNRSVRNQVARLAQKNYAVMVRDTFLVAVVVTVRLF
LATSPPDPFGLIVPPSRKSVFIEVTALPFHTSSEGDSPTTSSEPIKTNMYKDTYKSLNVI
PLGMLLYYIAVSDLIIKLLTMLVKLIITMLPHHLMRLKVRARLYVLVEYISQFYRAMTPI
TQWLLFLYESYSGLEVVSGGLFSAMYLGAKIFELVERGKSLKKAIVTFRKNIDSERPPTK
DELDAAGALCPICHDAFNTPTVLECGHIFCDECVQTWFKREQTCPMCRAKVSDDPAWQDG
STTFFHQLY

>Amphimedon queenslandica RNF183
MDSSQKPVVASVPPRPSNEVVNEASSSSLLCPRCNDSYTDPRLLSCLHSFCTPCLQSLVDGKASKQTVTC
PTCREITKLPKGDGGITSLPCNLHLISNQEDILLKITSSSTSPSCNSCDDGAGCAVAYCTDCMDFLCQTC
WGAHQTVKVTRSHSSFTLEKARAMPRGDLLKLLPSSSLSCHDHPRQELDFYCNQCSVPVCVKCTIISHRD
HTVTEAKPQAVKTKEELTKVMTAVHESEEKLKAVLDAGERTNEMIRKQKDEVDTLIRQSIASLQKLLEGR
EQSLIEKSNDVSLAKQTRLSRQLDTFKSLMEDMKACQDLSQTAVRDYGDVELLSIAHTLQARATELQEKF
SGTSLTLCESPSVNIEMNVSKIASGIASLGTVNGVDPICPEETTAEIPSCIHTVGKELQIKVISRDSTGK
RLNKGGARVTGQIACKGGGGGKKHVSATIIDSGDGTYTASFIPQQFGQQKITLSLNDGPIKNSPFNLYIS
RDYASISSLKGPLVTISDIDSPFFIAIADNGDVFVTSHFHHCVYVYSSNGDYKRVIGSKGKGQLQFLQPA
GIAISGNTVYVAENNGNRIQRFTRKGEFIDTFGSEGSGKGQMKYPWGICLDSNGKLYVTEYGNNRVQVFN
KDGSHSHFINATSNKMDRRFDTPEGVAIDASGRLHVTGFNSNNVAVFTLDGTFINKYPVKQSPCGIAFDP
VGNPLLSMNGGTSKIGVFDSSYNLIASFDKVFNVRGIAVANDGTIWVAEIGHSRLLNF

>Ciona intestinalis RNF223
MSPIRTRASSTPDYNIFKENIRFAGHMMASSIDLLTKINDEFLTCQICFETYTRPKSLNC
QHTFCLKCLEEYTPPNSVRVICPTCRSEQPLTADGINGLKDNFFISSMSDMLKTVKEIRS
EDKDGTSLMCDTCDHDNRKVAIARCLDCTDFLCNECSTWHIRTKLTRRHKIVSLSEFESG
IHNQELKSRAKIYCMIHDGEAAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQ
IENLLKTAKEKTDVLRTLQRTLKDIKQTKKLDRDKAESEIERICEIFINTIHRRKQELTD
ELHNMHATEVKRINADEDSVEMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQL
LETHPAATYHNTNQTDNIQFDTNADASVRVIRDNLGRLIIQKATFDEVVNKVLKQLDSFC
VADNHSMVRRNGKTLIQRAESTETKLVVQLGKQGIADGEFESTPNLAINSVNEVITADYD
GAKIQIFDPQGNFKDSFVTEVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGF
GKGQFGNCSSIAVNSENRIVVADVGKHCISVFTDTGKMLLQFGAQGKGESKLVEPRYVAC
DSQNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGLCTDEHDNILVADCWN
HRVDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLIISPTTLIPDFDQLAGQFDLPNL
LSNKVSWWLTSPNAQDPEHAVNGLTNGNIHNTIATSSITRKPSKTLASKKSSSSDSDLTA
MTPLSPIAPPSTPSLTGQQALDAVLNGTNGGSNGSLSSTGSGKIHYQGKVTSLNDHVPVT
MVNGT

>Ciona savignyi RNF223
MMASSIDLLTKINDEFLTCQICFETYTRPKSLNCQHTFCLKCLEEYTPPNSVSVMCPTCR
SEQPLTEDGINGLKDNFFISSMSDMLKTVKEIRSEDKDGTSLLRVGRLMLSESCGIHDGE
AAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQIENLLKTAKGKADVLRTLQK
SLKDIKQSKKRDRDNGEAEIERICEIFISTIHRRKQELTDELHNMHAAEVKRINADEDSV
EMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQLLETHPATTYHNTDQTDNIQF
DTNADASVRVIRDNLGRLTIQKATFDESINKVGWGDVINVGKLYTWFVLARRNGRTILQR
VESTETKLVVQVYQWEWAPMSTSIRVYTGKVGGSPLTLLMAINIMDIISTQVIVPSVVKT
TDVRFSKVKTINIWSRFMLLFCVQIGKQGIGDGEFESTPNLAVNSVNEIITSDYDGAKIQ
IFDPQGNFKDSFFVYNPQVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGFGK
GQFGNCSSICVNSENRIVVADVGKHCISVFTDTGKLLLQFGAQGKGESKLVEPRYVACDS
QNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGICTDENDNILVADCWNHR
VDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLVLSEDYSWSVKVFTLPEYLHESTIS
SLDEIISPTTLIPDFDQLAGQFDLPNLLSNKVSWWLTSPNSQDPDSAVHAMTNGNIHNTI
ATTSVTRKPSKPLTSKKSSSSDSDLTAMTPISPVSPSTPSLSSQQDALTNGAITGSNGSL
NSAGSGKMNFPGKVTTLNDHMPVAIVNGV

>Branchiostoma floridae RNF223
MASSLLSQISEDFLKCQICHDTFKRPKVLSCLHTFCLQCLKSYLKKNPRVSIKTFLCPLCRQETATPPTG
ADGLKDNFFVSNLLEALSTLKTFKVKGFVACGGCSRSASSRCMECEDFLCVHCHGVHAKLKVTRDHTVLS
IRELSSEGGSAQIRAYTKATLCPVHEGETMRFYCETCQKPICRDCTVLDHSKPDHKYVYFKDALSSRKDE
IQDLLTSCRGMVEGLEETKERLESEEGELRETKEGTLKDIEEWVAGLVLDTQRKKEELKDQVETIYNEQL
KELSAAKSRVEMTLNDIDSGCGFSEKVLDIGTDFEILSMRNEMAARLSDLTKMERPKVDQNRSGLIKFRP
YGGIGELVKLGVGELDSRTVGELVVKFGGWGPQQGQFKHPGGVAVSSAGHIIVADTGNHRIQVFDSRGVF
LRAFGFYGSADDAFSHPHDVAMTTDDRILVTDKGNKLVKLFTLEGKLIGKIGTGNLKEPTGVAVYKHGGV
AVTDTRDVKTFTRTGVMSATCTSDDPGYSHYLCTDDEGRCSKCSL

>Strongylocentrotus purpuratus RNF223
MAAKAVEKLVRELVCAVCMNTLKNPKSLSCRHTFCEECLKQCDEASQGRSGGIRCPMCREVTTLPRVGVA
GLKSNPLIYRVLEVLEAKKNSTQIICDACGDSESPLTTYCRDCLHFICDSCASSHSKMKLLMKDHRTVPL
DKIENGTIKIKWMEEGLWRKAHECEEHGGELRRFYCRTCKKRICRDCIVLDHTKPDHDCTTVQKMFQETK
EKLQDRLKTCEEKEEHVSTILNDVDARETGANQKFHKMKKSIRDQKSKAVDNLDKAEEELLQKVDVLEEK
LNVKLQAFRDNMNTTKENLTRAQSKAKEVVSAKEVTDISRGPFLPEDVLKELEELIASDVTMTISVDLVA
LKSTMIEGSKMPEQMARFAKLSLSRVWRETLMFPIPDDKDIVNIFSDGKAMYVACKKVIYKKLLSSEAKP
SIWLNLQLNASIKQVVDVAISMKDQTFSFIADTDAACTQLYRLKLEDFPKSDEHTTVPPCPLNPSARLTV
DSQSLIVVADNKPAISSSSQTTSSESEPVSETKQKKPTSSPVVEGSQTVSAVGSAKSLSKAEGTLSPSDS
KGKETRPEIPKAGSDIFTISTKIYRENTEKSTSQLSSDNFEVPLDRVRSLAAAKTGQLVFLRGDKKAVII
TDKDGGVQLEIKEPGRMYLSISCTESDALYVLSAFWGSHIVTLAKHSLEDGGPLEYIIDELDVSADFQSD
EWPVIGNYGNDLVIMHTGDGIRVFSAEDWDILDDKDANGQDQDDDKEEDV

>Apis mellifera RNF223
MEHNQATDINEIPKIVGGSVAKDGQYPYQASLRYRNRHFCGGSVLNERWILTAAHCLSSFNDTAITVVLG
TNTLDKGGDDYQSEKIIGHPRYSSILIRNDIGLIKLNKNIVFGDKVKPIQLPSENFNKIDYPAVLSGWGT
TSYPGQVPNELYYIQLNVIDQKQCLNVSFRVTNDNICTLNKRGEGACHGDSGGPLVADNVQIGVVSWGTP
CARGQPDVFTRVYSYIDWINIFYRTRLGERMVSMSSMLVETVSINYEDFNESFLTCGTCLCVYDGGEHTP
KLLPCSHTVCLHCLTRIAASQTRETGAFRCPICRELITIPRGGVPALPPSFLVNQLLDLMSRQRREVIPK
CSVHINQELLFCETCDTVFCTVCTGGNHAGTSPGCTEHTIIPFSIAIKRMSEILLYKANECISKLTQAQD
SVSTELQRLEASTEKCLNAVDTEFAEIISKIERRRSELQAAVTAAARDKKHVLEEQHALIEAEKNKVQGE
CEGLQYQVEVRNITQRIGSLSDQLDAASALSEPKENAFITFEFNHNNALSQLEEALNNLGRVRSSTTLPG
LCRARLKDPAIVKLQAAVIVETVDYHGHPRNVGGDLITAELTLADSIHSENQSSSIETEILDLENGTYEV
LFRPPSASRYILKLSVFERPIKDYPLFFDATEHNEPIKIYGRRGNGKDEFHQPVAVAVDDDGMIYILDTG
NSRIKVLNYDLEFQRHITNEGLEGRSCTGIGISQQGIVVVNWRTRKITEMSSLGDTIKSFSHNAFQEPID
VAVDRSYGHILVADNGQSCVFVFDSDGKILFQVGKRSTFKLITSVTVGPNGEIIVADSRIQVFSAKGDFS
EEIYSEGKGKGTYGGLAVDAEGRILGTRTDKGRSIIQVLKLGGGNILTEIDSHSSKLRRPSGIAVLPDNH
LVVVDLGNDCIKKYRYW

>Anopheles gambiae RNF223
MYSFSTSSKSGANSGGSSLVSSTTSSSGGSSTVSTSSIVGGEVAVTAASASVGATIQSMVASVVADPPEL
VAGAVERTPGAGCLPAVSSAAATSTALTSSAAVAILQQAPPASGAVLSASPRADQLVPKLGGPSPNNSSS
SVCSNGSSTSSGMFPEVFKLDDMLELENVGNGALSENGSTGGDFQLDNSSLSDLMEQDCTLCKNKFTSPR
VLSCLHVFCESCLDKLLAEGAGDKGLDGGNGGTIVCTICKQATPTGPKGAAGLHQDYILSNVLDLSTIEP
ALLACTSCKSKEMAISRCNDCANFLCASCEYAHQHMRCFEDHKVVQLADLRKSAEKVAIHKPLYCPVHSM
ENLKYYCFNCQTPVCNECLIGDHKGSEHNYHIISDAEKPMRQELECSMREAKSKIEYCNQATSKLESSLH
DLQSQYETARGLINESYQSCKAVLEQCRENALKNLEKLHSERELKIMDLYDNVAKSVEKIEVAAKYARKV
VDQANGPEMLSMKSMICAQLNQVMVTAPKVDVSFSIEFQSNYEKFEQLVPELFGKFRTESSLPVSVNETT
PSPTLPGVPIMVADAHHQPVNGGCGLSQGPLTASVTASSPISLPTSMQSSFDGDISTLGTTYMLPNVLTP
EPATGAANIAGPPNALSAGTPSPGVVVGTGPATTLPGLSSIAEYNLHRLANLAGETASNDLTDSIVPVVN
SNPATGPAGGATPNFTLADLISGNQHAIQALAKYSMGGQDMSNGAMLSAHPSLDSVPMMSDFPGVLPGST
SPILPVPPNMPNIDGPGMNGAGSDMAMSRFQPSMCVNGRTKATPMQIRTKFGSLGQTKGQFNSPHGFCLG
VDEEIVVADTNNHRIEIFEKNGTFKFSFGVPGKEEGQLFYPRKVAVMRTSAKFVVCDRGNERSRMQIFSK
NGHFIKKIAIRYIDIVAGLAVTNKGLIVAVDSVSPTVFIICEDGNLIHWFDCSDFMREPSDIAINGTDFF
VCDFKGHCVAVFSEEGTFKYRIGSEKITCFPNGIDISDAGDVLIGDSHGNRFHVACYSKDGQLQSEFECP
YVKVSRCCGLKITSEGYVVTLAKNNHHVLVLNTLYIQ

>Caenorhabditis elegans RNF223
MDPFDIDGLNVRLRDLQRQLQDDMEEFRDMAQMDDFAHPFGGIDAAINVDDPVDDANNDE
PAAGAENVLDREDNQPQPEAVATPDVPKAENEKEKEDEADDTILTSKGTEMKGLSCGICY
DPFNTGKRIPKVFPCGHTICLQCIKKLLNTRTFLGGNTVICPSCRQNTRYSLTTPADKIP
TNFCIQSLLEQRQQEKSTKEPEAVKCTECHTTVSAIEATTCSDETCQKEISEEELKNVDK
IKRPKETLICRSCIETNHSTHIFVSFSNVAAQYEANKKILLAESLLKKKLKAVDEALLAF
DKTKAEAIDHRRRMANALVNIRKEADHPDFYESLNRFMSSMKSSGELFKTLSSDLDKFEN
SSKARYHRSFGRDVLRPSAFPQETDGSKQEVKQAPAPVPVPPARPRPGPALNRERFRRLG
AVGGFVVPIVGRDANAPGGVRHLANEIDEFLAPNDQGLAAHFRRIFGDLPDDDIAIDVQI
AAAAPAGDAPAAGGGDDAVDGAGDFLGPAADLRLAGAAGAPLNIADRMYRRHRIAARAQL
AAPRRANRNGNNVRDLRMVRHHIFRAIAQNPGLHRNDAIQQAIDGLRDYIWNLEGPYARN
PFELDQIDMRRDLDNAQQRLNQLGARLQELEGNLRARDDPAQDAPWHDVVVQEPLNPLPP
IQGIEQEVADRAEIAELVNRIMGMVRNEAEAEANANGQRAPKNIEMPALLNPDANPDAPN
FKNLQPLQVQPYLRDLRALELEWIQKAQDRRARGGAQPPVEDVEQNPVVEGGTDAQRRAV
SPTQDEPAAKRNRQ


>Nematostella vectensis RNF223
MAQTYPNKTTSLERHALKISKKTNTKGYERKARPSQATPPIIKSKTLRVSKQSSLKKLQRALNDELRCSV
CYEVFSDPRTLTACLHSFCKECLHKMLSKRSKYIHCPLCRKKTAVPRRGVKGLPLNSVIRRLVDVHSSEG
MTSARHRKRHPGIK

>Amphimedon queenslandica RNF223
MAARANADISEILTCSVCFDDYKDPRTISCGHSFCLRCLESISDGKMVFECPVCRTETAVPEGGVSQYII
TFALKSLKDAVERTEGEEEYHFYCEKCRELLTYQTAATTHQGHKYHNIQVSYANHLEALKKSLICLRART
EDVAADREVLLQVESDILKQKEQIREEIVTVSDEIINAVRKATQQKLAQLDKIFDKKMETATQQRQESDE
MLKTFVGIQEEVEHKLSTSSCCQLLSKKKEISEQIHSAMTKEKNFKPKEKANGKFVRNSSLVSCSQNIGY
LFPKSSPRYSVKHDPVIMAGRPSTFTVSFSPLYPPYDSSPPIKCTLTSCDTRNATTTVCDVGKVQGFSHS
FEVVPIDRGEQLLTITTDDMLTAQKHDTFIAHPSPHMRGRPLKEIKNLKKPIGIAITKNGGLIVVNQGSN
SIEFINDTGRVSRDIKFTDPHGIALTDDEAYFVVTDKHSVRLIKKQSGNVIAIAGGEKKGYKRNLFNNPT
GVAINPMTKQIYVADTDNHRIVVLNEMALTRVTEFGKKGSSSGTFDEPMGMEFNTDKLYIVNNGSSCIDV
YSAIDHNPLFRIGASPSPLSYEGLSCPSSLTIDAFGYMYITEVTAPIFSFKSNAISRISIYKTDGQFVRS
FGGKGRTEESFYDPSGIAIDRRDGTLYVCDTDNNRIVLL

>Ciona intestinalis RNF225
MAGAKSLKNKLEDDLLTCPICCSLYNDPRVLKCQHSFCHGCLKGSMKAAKSNGKSINCAV
CRKPTPLAKKGLADLPKNYVIIGLSQMVKEHKESGKSPVVAKSSSGKNVGNVKQLVETFN
KKSIVEKPSKKRDKNFNIEVPKAGVVHDDENDDSSDLSTSASDSSGMTEFTPTDDSESTS
SGSDDSSSGSDDSDTDSESDSGSSDDEEPEHVPQRPTIKSSHGIKLGMKVFVFTAKGKPL
PGTVRVLEKYKRKPPNQTQVYAGVQMEKEYKKLTTEDILDMDDSYLFNWLMDFDQPCVAV
RIDQCCPAKVIQRFNL

>Ciona savignyi RNF225
MASASKSFKNKLEDDLLTCPICCSLYSDPRVLKCQHSFCRSCLERSVKASRTNCNSINCA
VCRKTTPLGRKRLQELPKNYVIIGLSQMVKDHKGNGGSSVDAEEPSMKENVVDVQQLVKV
FNKKSISSKKKSGKDKNDNKLIPNVMDGGDDSSDFSTSASDFSSEFTPTDSDQSGSSGSS
SGSSGSSGSSSASTESSSEDCDDSPEVLPPTPTMKSSLGIKIGMKVYVFSSRGKPHPGVV
RVLEKFKRKPPGQTRVYAGVQLEKENTRLTTDDIEDMDDSYLFNWLMDFDEPCTVVRVDQ
CCPAKIIESVMK

>Branchiostoma floridae RNF225
MATGGESPQGSLQIENFECSICLQVYMRPKVLSCGHTFCKECLVPLAKGSKSFKCPTCQATIRLERAGRA
GIEGLIDNISLSNLRDYVETSNRQGPRGSENPTVVSDESQCPKHHGEELRYYCPPCDAAICEECMFEDHN
THGVTRLRRTLQEQSIKAKEVCEEGTRLIETIRHKITELEQSRKRLAVEEGTLVKAVDQTARKLADDFAT
EVTKRANSLKDELHNLFAPSNSLVLDCKESLEIRLAQLVSSVESLEKSINQGEAIVVFSGRKMLKDLIEQ
SSQPTKAEQDVETGNIAVFTPTPYSFPAITLGKVQATSKIWERKKETDEEKVETSAAPKEDSPAPEVDGV
TNNNEAEENDEDIVEIEDKSGLKYKCREDQRNPLNSEGKKQYDRSFLLQFQPECIDKPDGLPKIVDVVLD
KPLIITPQQMQGRPQDFRGMGGSPGIPDPWPIPSYMKSPGGDHRRMEVFEKMQGILDKLTSQNFQTLCQQ
VLDLDIVTEERLKGVIDLVFEKAITEPNFSVAYANMCRCLIHLKVPMKDNPKQTVNFRKLLLNRCQGEFE
RDIDNKMEKDERMRKIELAKNEDEKKKMQAELEEEMANASRRSLGNIRFIGELFKLKILAENIMHDCVVK
LIKFNDEESLECLCRLLTMIGKDLDVERAKPLMDQYFEQLERNTKQKKTSSRLRFMIQDVLELRRVGIPQ
SLK

>Strongylocentrotus purpuratus RNF225
MADALKNAIAQSLECPVCLNTFTDPKILSCSHTYCKTCLDNLLDCHGNDQMLRCPVCRAETQVPNQEVSK
LPTNLALKSLIEDVKNHYQFCTNCKSEDKPQAVVYCQDCGKYFCSTCHNKHSQWPDFISHDVLAMSEIVS
GKVSVRRYRKCRKHPKDDEEWFCSDCRRFACFKCVVMEHTNEGHRVIEAAVYESNHMKSIEDLKSKADKK
RSCFQKYIDFIDEQKEHVSNVQKQCTDDINKAYEESVRQLTKKKEVLIGEVKGRTEGVEKELDEIKKSAQ
EHITHFTTIADVVTNRTKIPLDTDALAAHDTLCQDLQEALKQEDPDYKQSIQLSRKGKSVSFKRNVGMDE
LALGKIANAFAFKNIALPTKNSMNIMVGTPDGRMAVGCDTGGVEIFSADGQLQQTVLKGVKIRGVGFLSD
DRYVILDCSNNISLYRLQYTKLNVVFETLYNDEGEWAILNVGGDDQIYVSYWKAQKIQVFSPAGGKAIRE
IPCDGYEPQQITSYNDYLVTRSWDAIRLIDKEGVVKYELRELGYFPYATVSQGNTILIAKVNHDVGLVSI
DEYTNELKHIQNLVFDYKIEKPERWWYYLQQYRSGEIAFCTPDRLYIFHWK

>Anopheles gambiae RNF225
MSTNGWIHCNICFHSFERKERKFYHLSCRHVLCKLCMAKTNRGTMCPLCKKPLERFTELNNQMERKEKMY
YDPGSLKVFGVAYQSVIFQHKQREDMIRGILRCRSAVTQMKEMENAMRQKIVETQRRYEKFRTHRRNLQE
GLRQISPRYGSGPTVVQGAGCAGRTPLQPLTGNRASPQLALANRDGDPASRRYQDKGRPETVTFAASPES
SSADFSFGTPAPSNRSATVTDGSFTPAEQQHARLSTPQVSTERFFREGNKPAVTGQGDGVFADDSGISSM
QTPTSSLSFTGSSRMAQGMQQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQQQHQQLK
QQRQHPYTPSTPARHLPGTSSYRHSQIFPPTVPRP

>Caenorhabditis elegans RNF225
MSEKNTENPEILSTVLAGYCQCQVCYQPFNETTKLARSLHCGHTFCTECIRNVQNYGNSP
HLECPTCRAETKSNIENVAPNFSIMELARNFGLLGPPVDAPRTSSSCCSNKTNRDEMIDN
KFRELLVEVKSSLIHHFDVFRIKSQLWSLAEAEREVSNMAEALAGAVSECYYDSDDNSDA
NSEDQRVRTMLRFLAARDSDSETTEDSTDFLESNTTVPDRTLGGGFYRNFRRLFGDENEL
DDPGVVSGIEEAAAYRRFRGLFGNDNDSISQVSTTSGSFGNHENDHFCPFSDDDNISECS
DDYYPTLVMPETDEEETYKSFRCLFGDENGNLDFSDDEKEFSDNESSLSSVSTEDFPNSH
IEEAGRVLHRFLLLSALLNEE

>Nematostella vectensis RNF225
MATVRDEGYSPQSLNCRACHKVFTEPKILDCLHTFCQKCLGTHDILGAGTNSIVCPLCRKPTPIPESGVE
SLPSNFLLNNALDQLSVKSSKEYILHCTNCEDASDATQTLHRPSFCMQHKDEMFMFYCLECEGLICRECT
ILDHRGHKYAGLKDALEGRKILIDDLLGFCTVKKIPPVQDAVYEVKAMTAKLRARADAVKNDISSAADEC
VKRILARKQELLTQVDNMTQSKEGVLQAQQEQLEMELFRYTSSRDFVENVLLHGNEVEIIQLRQLMTNRL
NELNGVKLDYKEPEENDVIDYILDSEAVQKVAMTLGHVTTSQTFASLCIAKGLGVMSGKVGVESFFTIET
KDRFGNTTFEGDHCTPIQVIIQAPEGFFLGNHVANKRDGTLLVRYTPVTRGKHVIVVSIRGRQIEGSPFT
VKVVEGIDYKNVMAVFMKIGSQGTKSGEFRQPSSVATDTDGNIYVTDFVNCRVQKFDMFGKHLKDIGTKG
SKDGQLMNPCGVVVGSDGTLIVSDWDNHRVQMFSPEGKFVSKIGRKGSENGNFLHPSGLALNQDGDLVVI
DKDNNRVQVLKLDGSHVMSFGSLGNADGQLDCPSHVAVTPDNGYLVTDTGNNRIVKFDASGNHVMSFGTK
GSGEGRLDRPAGIAIDPEGFIIISDFQNHRVQVHSPEGEFYATFGSEGNIEGQFKFPSGIALTLDGHVIV
ADRHNHRIQVF

>Hydra magnipapillata RNF225
MAALSSSQVLENIHEQLVCAICLERFDDPKLLNCLHSFCCQCLHKIINQKPSDVIECPTCREVTKIPING
LKELKTNFFINSLLDLVHHKIEELKNEKKKGEQCEQCQEHDNATSRCIECDLVLCGGCIADHRRNRSTLD
HRIVVMEDDEDVNKQSLDKYTLTFCRYHTRNVIKYFCNTCDEAVCRVCTILEHREHQFVYPKDAIPQQKP
IIEELLEKTKENIPLLKATIKDIHSMSNTLRECRESLLLEINAKTQARIEALLLAKAKLLEELDRIHRGK
QKTLSLQSDAVELELGKISGCCAFAENVLKFGNEVEVLQIKSRLQQLNSITIKLDPEEDDSIQYVSESES
IEAVMESLGKICSSQTFASLSYAEGEGLETPRVGIESNIIVHAQNRHGKKKSGSDRVEAVMVLPDETKRT
LNALDNDDGTYTIRFTPDINGRHLLHITIKTKPINGSPFEIFVINRREYSEVGPSLLKFGQYGSKRREFK
SPFGVASDNEGFIYVADSYNHRIQTFGPRGEFVHMFGSHGDRKGEFNCPTDVAVSPNSKLVVCDNGNNRI
QVFGRNGNFIAKFGREGIKNGNFKSPWGVTVSPVSREIIVADTDNHRIQIFSSDGKFIMKFGCNGDRPGQ
FNSPCYVAANAENEHILVSDSKNHRIQIFDRRGKFIQLFGTQGQEDGQFNHPRGLSMDIANNIIVSDMGN
HRLQILTYAGKMVKCVGTEGNGDGQLSFPESVAVTPNGFIIVSDLSNNRIQVF

>Amphimedon queenslandica RNF225
MTLEEAPERDLFEELEENALTCAVCLGVYEDPRALDCHHVFCRSCLEHLPLVNQDGVNNLGCPTCRHLVP
LPPNGVAGFRVSFQTNNLKAFVEANRDRAVPVAVPRKQTCGSCTSENVSGFCTECRSTFCDGCTDLHLPT
CTKEATALNGASGTDKTNGHHQAPMPLDPAPLMQSDDWNVNTNTCVIHRQIADMFCENCEETICSRCVKR
FPHTEHESSCIPLAEALVRYRELLEDRLNPINKEIDFISTQLKPFSHRESEINEQGDTVKAQIHSRAEYL
IALIIESEEELSVKVDKVIKSKLHSLDERRESIEVTQEELKNLKDRIELCLKTGNQEDLLKTKQWIPKIN
HVMFNIDATKLKPSEVADLSFVPDEVSDSLTRLGNLSSTFEGKDPSCDVQILEQHLFIDKSPDVTLVLSV
KYTNLPFVIIPLSSMKCIIKSSNRSFIKAIISLGDAAHQYVVKFRPTSNGLYKLSLKIDDIETEDQAVLI
PFNPMYLPNITPTTVMKYIRKPIGLAVAQDKRIIVLNDFNEVVILDMHGRNIRTLSRPNNGSRFTSTRHA
AVTSDGCFLMTDQYSILKVTMDGQLLKTIGSKGNGRLQFNTPFGITIDEKTGKVYVADLLNHRIQVLNRD
YSFSHSFGSRGKALGQFEQPNGTALDSQGNVFVSDTFNHRIQKFSQSGEYISYFGSEGPNPGQLKLPEKI
VINNDYIYVTEHLNARVSVFTTEGNFVRCFGKQSIEGGLVWPRGLTFDEDGSLYVCDFRSHCIFKY