>Homo sapiens RNF183
MAEQQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLALLRLEPHHVILEGHQLCLKDQPKSRYFLRQPQVYTLDL
GPQPGGQTGPPPDTASATVSTPILIPSHHSLRECFRNPQFRIFAYLMAVILSVTLLLIFS
IFWTKQFLWGVG

>Pan troglodytes RNF183
MAEQQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHLSLVTPARRCLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPRVYTLDL
GPQPGGQTGPPPDTASATVSTPILIPSHHSLRECFRNPQFRIFAYLMAVILSVTLLLIFS
IFWTKQFLWGVG

>Gorilla gorilla RNF183
MAEQQGRELEAECPVCWNPFNNTFHTPKMLNCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPRVYTLDL
GPQPEGQIGPPPDMASVTVSTPILIPSHHSLRECFRNPQFRIFVYLMAIILSVTLLLIFS
IFWTKQFLWGVG

>Macaca mulatta RNF183
MAEQEGRELEAECPICWNPFNNTFHTPKMLDCCHSFCVECLAHLSLVTPAQRRLLCPLCR
QPTVLALGQPVTDLPTDTAMLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPRVYTLDL
GPQPGGQTGPPPDTASATVPMPIPIPSHYSPRECFRNPQFRIFAYLMTVILSVTLLLIFS
IFWTKQFLWGVG

>Callithrix jacchus RNF183
MAEQQGRELEAECPVCWNPFNNTFHTPKMLDCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPRVYTLDL
GPQSGGQTGPPPDTASATVPTPIPIPSHYSLRECFRNPQFRIFAYLMAVILSVTLLLIFS
IFWTKQFLWGVG

>Mus musculus RNF183
MSEPQGQELRAECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDL
GAEPGSQTGLPQDTAPDTRPVPIPSHYSLRECVRNPHFRIFAYLMAVILSVTLLLIFSIF
WTKQFFWGMG

>Rattus norvegicus RNF183
MSEPQVQELKAECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVSPARRRLLCPLCR
QTTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDL
GPEPGSQTGLPQDTAPDTRPVPIPSHYSLRECVRNPHFRIFAYLMAVILSVTLLLIFSIF
WTKQLFWGMG

>Oryctolagus cuniculus RNF183
MAEQQGRPLEAECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVTRARRRLLCPLCR
QPTVLASGQPVTDLPTDTALLTLLRLEPHHVMLDGRQLCLRDQPKSRYFLRQPRVYTLDL
GPEPGTQTQDVASQDVAPATASVPIPRPRHDSLRRCFRNPQFRIFAYLMAVILSVTLLLI
FSIFWTRQFLWGVG

>Felis catus RNF183
MAEQQGREPECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVTPARRRLLCPLCRQP
TVLASGQPVTDLPTDTAVLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDLGP
EPGGQTGPPQDAGPSPRSLPVPVPSHYSLRECFRNPQFRIFAYLMAVILSVTLLLIFSVF
WTKQFLWGVG

>Bos taurus RNF183
MAEQQGREPECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHISLVTPTRRRLLCPLCRHP
TVLASGQPVTDLPTDTAVLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDLGP
EPASQAGQPQDVGPSTRPVPIRSRYSLRECFRNPHFRIFAYMMAVILCGTVLFIFSIFCT
RRFFWGVG

>Equus caballus RNF183
MAEQQGREPEAECPVCWNPFNNTFHTPKVLDCCHSFCLECLAHLSLVTPARRQLLCPLCR
QPTVLASGQPVTDLPTDIAMLTLLRLEPHHVILEGRQLCLKDHPKSRYFLRQPQVYTLDL
GPEPVNQTGPLQDMASATRPMPISIPSHYSLRECSRNPQFRIFAYLMAVILSVMLLLIFS
IFWTKQFLWGVG

>Loxodonta africana RNF183
MAEQRGREPEAECPVCWNPFNNTFHTPKMLNCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGRQLWLKDQPKSRYFLRQPRVYTLDL
GPEPGSQARPPQDMAPATVTMPTPIPSHYSLRECFRNPQFRMFAYLMAVILSVTLLLIFS
IFWTKKFLWGAG

>Dasypus novemcinctus RNF183
MAEPPGREPEAECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVTPARRRLLCPLCR
QPTVLASGQPVTDLPTDTAVLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPRVYTLDL
GPEPGSQAGHPQDAAPATRPGPVSIPSRYSLRECFRNPQFRIFAYLMAVILSVTVLLVFS
IFWTKQFIWGVG

>Myotis lucifugus RNF183
MAGQPPEDAAESQVSDELECKICYNRYNLKQRKPKVLECCHRVCAKCLYKIIDFGDSPQG
VIVCPFCRFETCLPDDEVSSLPDDNNILVNLTCGSKGKKCLPENPTELLLTPKRLASLVS
PSHTSSNCLVITIMEVQRESSPSLSSTPVVEFYRPASFDPVTTVSHNWTVWNCTSLLFQT
SIRVLVWLLGLLYFSSLPLGIYLLVSKKVTLGVIFVSLVPSSLVILMVYGFCQCVCHEFL
DCMAPS

>Pteropus vampyrus RNF183
MAVQQSREPECPVCWNPFNNMFHTPKVLDCCHSFCVECLAHLSLVTPARRRLLCPLCRQP
TVLASGQPVTDLPTDTAMLTLLRLEPHHVILEGHQLCLKDQPKSRYFLRQPRVYTLDLGP
EPGSQTEAPQARAPTTTPIPIPSHYSLRECFRNPQFRIFAYLMAVILSVTLLLIFSIFWT
KEFLGGMG

>Monodelphis domestica RNF183
MAEKLGKEPEAECPVCWNSFNNTFRTPKLLDCRHSFCIECLAHLSLVSSSRHRLLCPLCR
HPTVLASDRPVTDLPTDAAILTLLRLEPNHIILEGRHLCFKDERKSRYFLRQPRVYTLNL
GVEPESQAGPPQQGAAPPAPVPIPSHLSLRECFRNPQFRIFTYLMAVILSVALLLIFSIF
WTKQFLWGTG

>Erinaceus europaeus RNF183
MAELQDREPEAECPVCWNPFNNTFHTPKVLDCCHSFCVECLAHLSLVTPVRRRLLCPLCR
QPTVLASGQPVTDLPTDTAMLTLLRLEPHHIILEGRQLCLKDHPKSRYFLRQPRVYTLDL
GLEPGRQTGFPQNMVPATRPMPISIPSHYSLRECFRNPQFRIFAYLMAVILSVTLLLIFS
IFWTKQFLWGMG

>Ornithorhynchus anatinus RNF183
SVAEGAAPAAAAAGGGEGGGEEAAAANAPYEDYECKICYNYFDLERRAPKLLECLHTFCQ
ECLSQLHLRGAAQQQLACPVCRHRTALPDSRVHGLPNNNKEKGIIIRRYDVLEGTKDTAM
ENTIKKKEEYIIARKKEAGRPGAAPPRRTGPSARTPPPWTPGPAWTAYESCHNCKRAALT
AGCVCVVFSFLAMVVLLFTGLIFVNHGSPVPSPVGPICLSVASILALFSVVVTWLICWLK
YRPEAAAAAAGGGAGPGRGR

>Anolis carolinensis RNF183
MSCFTQIWRTEVPESPPPSPTSPVPAAPHEIPIPLSPGSPTDRPTSPIECSICFNTYDNL
FKTPKVLRCNHTFCLECLARLAAARPRSCLEDELPCPFCRQITEIPLEGPPGLQTSKDLM
ATLPPEHQREKMIWMEGTKICCRQSSDPENADACISIDIAPSKPEVPQLPPETFMGRLTR
CDMCDDWKRVLLLSVLIIILFCIILWPLHCAMKTGNLRC

>Pelodiscus sinensis RNF183
MAEGPRGEPEADCPVCWNPYDNTFRTPKLLECRHSFCIECLAHLSLVSSARNRLHCPLCR
HLTVLPSDQPVTELPTNAAVLRLVQLGHHPXSSLQQCLRNPQLRTFAYLMAIILGVTLLL
IVFIFGPKQFPWGIG

>Xenopus tropicalis RNF183
MADAVKLDPDWDCPVCWNPYNPTSRTPKLLHCNHCFCLECLERLSQASPMRIPCPLCRHP
TVLPEGQGVPALPTHSAILSQVRAEQPTPRPFFCPKDSLKLPLFQRPPSIYTVSVGEEPG
PPFNSQSPLTLPSTGPPWHCFRNPQLRMFSYLMLVIAGVTLFLIFSIFWTRKFIWGQR

>Danio rerio RNF183 b
MGDRREKPQSSRKGSNAKPKLESKGSLNLGSSEQRQRADKPVRRSRSNDTENRRREGSRG
RERDRRDEGRPQRGRSEEGRRRGHNHEPGNRAWDKERPKDELQDTECIVCFCNFDNVFKT
PKLLSCGHTFCLECLARINVNSPEIKTLTCPICREVTEIRHGRDLPHLLNNEAIFRQLPS
EMQRALSVRFKRSKGKLVLKNPPTNNSIKTTLNLPVLKKKEEQAGNNAIGAIEEGLGQAT
MINVGRPPNRMRGRMRRVMYSSQCYYAVVAVIISITVALMMVGIFTFVVMPNLQRPITKP
LQNQTGSSAVQADSSP

>Takifugu rubripes RNF183 a
PGGSQSQSQQTRPAVNVKPKPTQKEIRYSQTQWKKEKPAKVRRSRSSDSERVERGRRSNK
ERHHGERRRRGRSEETHRPDRRENRDEGSKAGPPPDDMEDTECAVCFCTYDNVFKTPKLL
ACGHTFCLECLARINVTSAELKTLSCPICRELTKLPHGKDLPRLGNNLDIIDKLPPDMHR
ALSIRFQRSKGKLLLKNPPPTSPTKSNLLTLPVKSQGGPVTPEGNLDAMEQGIVPTTVVD
VGRPPSRIRGRLRRVFRSDQCYYAVVGSIITITVVLMLVGLLAFVIIPKVPVFQSPTPAG
NHTSGHTGASGGSSP

>Takifugu rubripes RNF183 b
MDTVKIIQSEKFPRECPAPVTQPRFAPPPRVAWDGGGEGEIIVNQACSDLTLDMAGSPRP
MVPQVAPVLRRDPSFLAPRKTSANEICYHQFHYKMEDVIVNQYVLRPVMPCEPLDCPTCG
HTYNFTGKRPRILSCLHSVCEECLQILYESCPKYKFISCPTCRRETVLFTDYGLAALAIN
TSILSRLPADPNGPVQWGGDADRSCYQTVRQYCQSACTCHIANPLSTCGIM

>Tetraodon nigroviridis RNF183 a
ECSICYTTYDNVFKTPKELDCTHTFCLECLSRLMALSPADQDSDHPLKHISCPLCRRTTT
LPKEGPPALTTNHEVLRKLPHHQQ

>Tetraodon nigroviridis RNF183 b
QSQSQSQSQQPRPAANVKPKPPQKEIRHSQTSWKKEKPAKARRSRSSDSERGRRRDSERH
HGERRRRGRSEETQRRDRGGGGTERSRAGPPQDDIEDTECAVCFCTYDNVFKTPKLLACG
HTFCLECLARINVTSAELKTLSCPICREVTRLPHGQDLPRLGNNLDIIDKLPPDMHRALS
IRFKRSKGKLLLKNPPATSPTKASTLTLPVKSQGDAGVHPEGSPGAMEQGMVSATVVDVG
RPPSRIRGRMRRLFRSDQCYYAVVGSIITLTVLLMLVGLLAFVIIPKV

>Oryzias latipes RNF183 b
MAKDDMAEVELPSTGAEPPGEASSAFPYEEYECKICYNYFDLDRRAPKILECLHTFCEEC
LNTLHLREERPWRISCPVCRHRTPVPDYRIQNLPNNTKVTEDFPLYIDSDPLPQDALPPF
PPPLHPALVALRREEASGASSVSQATPSTTVSTATTLSQDSVRYDSCQSCKRVALTTGCV
CVIFSFLSMLVLLFMGLIFVHSHSIPPSPAGPICLSVASILAMFSVVVTWLICWLKYRPD
HETGRASSTGNSRRNA
>Ciona intestinalis RNF183
MAMKYKSSDSRKVPAQPPQWNQFLICSVCENEFNRTDRCPISLGCGHTVCRGCLGDLKHP
QCQFDQNSITCDISDLPVNSALLLLVPEEESHKGSVEMRGVSQKGKENFHPGNIAQCVKL
YDKSKKHIEELALLLRPNKGNELSRPMQRKLVALINCQLVEEEGRKRALRAGRALGERSA
TELILLHQNPTTLSASLWAAVRARGCQFLGPAMQE

>Ciona savignyi RNF183
MENRRTHIEASDVDLALEEAGHCGLYHAWLILLMAVPNILVGAHMGSSVFEAMIPTHYCV
TQQADDITTACNITWNPTNDALAVPEDLDGKHSPCHMYYYDNVTDIDCKFWTLDKNSTPT
VEPGIRTCDHWIFD

>Branchiostoma floridae RNF183
MELQCGLCGQQYSRPRVLSCLHTFCLACLESLMKRQDREGEVCCPVCQQRTLLPSSDPSTLPSDPIIDAL
LEVKSVEAGAVTCTSCEENSSAVCRCLDCAAFLCRNCQGAHRFMKCFKSHRLVELENLSISDLASPLKLL
TCTKHTGEFLEFYCNTCDALVCLKCTAIDHRRTSHQYVYVKDAAAVMKERMLYLLENGRKRSEEVRVQLL
RMEEKIDHVEVEKMKVKGEVDKVFHTLLSKLEDRQEEMCKEVETLYLRQKQFLNKQGKSLQLDEERLQAG
TRFVEKLMTYGSTSDLLTCGKLVTSQFDLLFKQCPGNDMKSSGAVGVSVDYHAFGNLLSRIQIQIKTVPK
IGVSSPVTQAGCSAVGESHRVESGSAEATPPSPKEQFSPSSSRSNLSEERKGSDSAQMDVTELHKVRSFG
KRENAGNCPYLTCVAVGANGNIYALDMYKSVVQVFDKEGKYCFNIGGRFQAPGRLEKPVELATSSKGYIA
VRESNGRMQIYSDTGQFIFKFQLLRVQRPRSMAIDRKGRFLVGDVGTDRCPHGVVIYSSDGKLLRKITKL
SAYTSSLAMSAQNHLLVVDHRAHCIQVYSEEGNHLRTIYPRGSRIYMRGIIVNQNKIILVTSEKVRNNMY
CGGLVVMAKDGTVLDKFKFPEKQPVVHVALTQDGCPLAILQGNSDICLYGSIYSK

>Drosophila melanogaster RNF183
MNNSPKDPYRGGGGGSGPQDGAGSTGVSINIESDDNDSTTAEHDYLLPASSSSVTSGGGV
GGGGVVSGGSTSTSVNLDHQHVPTARSSSLSGGNVRHGNILSTFLARQRSYTPASNSSSP
VRVTTQMNRRLQQSRSMGANTGGSLEEPSSAGAAGGPGASPGTAAGVRQMGFWRVNLNDV
FSLSTASSTEALRSFISHSNFRYPPATTAAPAAPGPQPAANPVDNSHILLGQQQPSMPEP
VSPLRYGRVSAVSGASSAGAMGRSISLREGEMRHNHSLNGGRHNASAIGLNSNPAAFDER
EANESQDLDESDGNVENGGGGGNPPEPAENNPEDEHLISDDMVVQILSHFVRYLPLIFIL
FFKFLHDHLLGIVDLLVLQTVMYNVNRSVRNQVARLAQKNYAVMVRDTFLVAVVVTVRLF
LATSPPDPFGLIVPPSRKSVFIEVTALPFHTSSEGDSPTTSSEPIKTNMYKDTYKSLNVI
PLGMLLYYIAVSDLIIKLLTMLVKLIITMLPHHLMRLKVRARLYVLVEYISQFYRAMTPI
TQWLLFLYESYSGLEVVSGGLFSAMYLGAKIFELVERGKSLKKAIVTFRKNIDSERPPTK
DELDAAGALCPICHDAFNTPTVLECGHIFCDECVQTWFKREQTCPMCRAKVSDDPAWQDG
STTFFHQLY

>Amphimedon queenslandica RNF183
MDSSQKPVVASVPPRPSNEVVNEASSSSLLCPRCNDSYTDPRLLSCLHSFCTPCLQSLVDGKASKQTVTC
PTCREITKLPKGDGGITSLPCNLHLISNQEDILLKITSSSTSPSCNSCDDGAGCAVAYCTDCMDFLCQTC
WGAHQTVKVTRSHSSFTLEKARAMPRGDLLKLLPSSSLSCHDHPRQELDFYCNQCSVPVCVKCTIISHRD
HTVTEAKPQAVKTKEELTKVMTAVHESEEKLKAVLDAGERTNEMIRKQKDEVDTLIRQSIASLQKLLEGR
EQSLIEKSNDVSLAKQTRLSRQLDTFKSLMEDMKACQDLSQTAVRDYGDVELLSIAHTLQARATELQEKF
SGTSLTLCESPSVNIEMNVSKIASGIASLGTVNGVDPICPEETTAEIPSCIHTVGKELQIKVISRDSTGK
RLNKGGARVTGQIACKGGGGGKKHVSATIIDSGDGTYTASFIPQQFGQQKITLSLNDGPIKNSPFNLYIS
RDYASISSLKGPLVTISDIDSPFFIAIADNGDVFVTSHFHHCVYVYSSNGDYKRVIGSKGKGQLQFLQPA
GIAISGNTVYVAENNGNRIQRFTRKGEFIDTFGSEGSGKGQMKYPWGICLDSNGKLYVTEYGNNRVQVFN
KDGSHSHFINATSNKMDRRFDTPEGVAIDASGRLHVTGFNSNNVAVFTLDGTFINKYPVKQSPCGIAFDP
VGNPLLSMNGGTSKIGVFDSSYNLIASFDKVFNVRGIAVANDGTIWVAEIGHSRLLNF

>Ciona intestinalis RNF223
MSPIRTRASSTPDYNIFKENIRFAGHMMASSIDLLTKINDEFLTCQICFETYTRPKSLNC
QHTFCLKCLEEYTPPNSVRVICPTCRSEQPLTADGINGLKDNFFISSMSDMLKTVKEIRS
EDKDGTSLMCDTCDHDNRKVAIARCLDCTDFLCNECSTWHIRTKLTRRHKIVSLSEFESG
IHNQELKSRAKIYCMIHDGEAAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQ
IENLLKTAKEKTDVLRTLQRTLKDIKQTKKLDRDKAESEIERICEIFINTIHRRKQELTD
ELHNMHATEVKRINADEDSVEMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQL
LETHPAATYHNTNQTDNIQFDTNADASVRVIRDNLGRLIIQKATFDEVVNKVLKQLDSFC
VADNHSMVRRNGKTLIQRAESTETKLVVQLGKQGIADGEFESTPNLAINSVNEVITADYD
GAKIQIFDPQGNFKDSFVTEVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGF
GKGQFGNCSSIAVNSENRIVVADVGKHCISVFTDTGKMLLQFGAQGKGESKLVEPRYVAC
DSQNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGLCTDEHDNILVADCWN
HRVDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLIISPTTLIPDFDQLAGQFDLPNL
LSNKVSWWLTSPNAQDPEHAVNGLTNGNIHNTIATSSITRKPSKTLASKKSSSSDSDLTA
MTPLSPIAPPSTPSLTGQQALDAVLNGTNGGSNGSLSSTGSGKIHYQGKVTSLNDHVPVT
MVNGT

>Ciona savignyi RNF223
MMASSIDLLTKINDEFLTCQICFETYTRPKSLNCQHTFCLKCLEEYTPPNSVSVMCPTCR
SEQPLTEDGINGLKDNFFISSMSDMLKTVKEIRSEDKDGTSLLRVGRLMLSESCGIHDGE
AAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQIENLLKTAKGKADVLRTLQK
SLKDIKQSKKRDRDNGEAEIERICEIFISTIHRRKQELTDELHNMHAAEVKRINADEDSV
EMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQLLETHPATTYHNTDQTDNIQF
DTNADASVRVIRDNLGRLTIQKATFDESINKVGWGDVINVGKLYTWFVLARRNGRTILQR
VESTETKLVVQVYQWEWAPMSTSIRVYTGKVGGSPLTLLMAINIMDIISTQVIVPSVVKT
TDVRFSKVKTINIWSRFMLLFCVQIGKQGIGDGEFESTPNLAVNSVNEIITSDYDGAKIQ
IFDPQGNFKDSFFVYNPQVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGFGK
GQFGNCSSICVNSENRIVVADVGKHCISVFTDTGKLLLQFGAQGKGESKLVEPRYVACDS
QNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGICTDENDNILVADCWNHR
VDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLVLSEDYSWSVKVFTLPEYLHESTIS
SLDEIISPTTLIPDFDQLAGQFDLPNLLSNKVSWWLTSPNSQDPDSAVHAMTNGNIHNTI
ATTSVTRKPSKPLTSKKSSSSDSDLTAMTPISPVSPSTPSLSSQQDALTNGAITGSNGSL
NSAGSGKMNFPGKVTTLNDHMPVAIVNGV

>Branchiostoma floridae RNF223
MASSLLSQISEDFLKCQICHDTFKRPKVLSCLHTFCLQCLKSYLKKNPRVSIKTFLCPLCRQETATPPTG
ADGLKDNFFVSNLLEALSTLKTFKVKGFVACGGCSRSASSRCMECEDFLCVHCHGVHAKLKVTRDHTVLS
IRELSSEGGSAQIRAYTKATLCPVHEGETMRFYCETCQKPICRDCTVLDHSKPDHKYVYFKDALSSRKDE
IQDLLTSCRGMVEGLEETKERLESEEGELRETKEGTLKDIEEWVAGLVLDTQRKKEELKDQVETIYNEQL
KELSAAKSRVEMTLNDIDSGCGFSEKVLDIGTDFEILSMRNEMAARLSDLTKMERPKVDQNRSGLIKFRP
YGGIGELVKLGVGELDSRTVGELVVKFGGWGPQQGQFKHPGGVAVSSAGHIIVADTGNHRIQVFDSRGVF
LRAFGFYGSADDAFSHPHDVAMTTDDRILVTDKGNKLVKLFTLEGKLIGKIGTGNLKEPTGVAVYKHGGV
AVTDTRDVKTFTRTGVMSATCTSDDPGYSHYLCTDDEGRCSKCSL

>Strongylocentrotus purpuratus RNF223
MAAKAVEKLVRELVCAVCMNTLKNPKSLSCRHTFCEECLKQCDEASQGRSGGIRCPMCREVTTLPRVGVA
GLKSNPLIYRVLEVLEAKKNSTQIICDACGDSESPLTTYCRDCLHFICDSCASSHSKMKLLMKDHRTVPL
DKIENGTIKIKWMEEGLWRKAHECEEHGGELRRFYCRTCKKRICRDCIVLDHTKPDHDCTTVQKMFQETK
EKLQDRLKTCEEKEEHVSTILNDVDARETGANQKFHKMKKSIRDQKSKAVDNLDKAEEELLQKVDVLEEK
LNVKLQAFRDNMNTTKENLTRAQSKAKEVVSAKEVTDISRGPFLPEDVLKELEELIASDVTMTISVDLVA
LKSTMIEGSKMPEQMARFAKLSLSRVWRETLMFPIPDDKDIVNIFSDGKAMYVACKKVIYKKLLSSEAKP
SIWLNLQLNASIKQVVDVAISMKDQTFSFIADTDAACTQLYRLKLEDFPKSDEHTTVPPCPLNPSARLTV
DSQSLIVVADNKPAISSSSQTTSSESEPVSETKQKKPTSSPVVEGSQTVSAVGSAKSLSKAEGTLSPSDS
KGKETRPEIPKAGSDIFTISTKIYRENTEKSTSQLSSDNFEVPLDRVRSLAAAKTGQLVFLRGDKKAVII
TDKDGGVQLEIKEPGRMYLSISCTESDALYVLSAFWGSHIVTLAKHSLEDGGPLEYIIDELDVSADFQSD
EWPVIGNYGNDLVIMHTGDGIRVFSAEDWDILDDKDANGQDQDDDKEEDV

>Apis mellifera RNF223
MEHNQATDINEIPKIVGGSVAKDGQYPYQASLRYRNRHFCGGSVLNERWILTAAHCLSSFNDTAITVVLG
TNTLDKGGDDYQSEKIIGHPRYSSILIRNDIGLIKLNKNIVFGDKVKPIQLPSENFNKIDYPAVLSGWGT
TSYPGQVPNELYYIQLNVIDQKQCLNVSFRVTNDNICTLNKRGEGACHGDSGGPLVADNVQIGVVSWGTP
CARGQPDVFTRVYSYIDWINIFYRTRLGERMVSMSSMLVETVSINYEDFNESFLTCGTCLCVYDGGEHTP
KLLPCSHTVCLHCLTRIAASQTRETGAFRCPICRELITIPRGGVPALPPSFLVNQLLDLMSRQRREVIPK
CSVHINQELLFCETCDTVFCTVCTGGNHAGTSPGCTEHTIIPFSIAIKRMSEILLYKANECISKLTQAQD
SVSTELQRLEASTEKCLNAVDTEFAEIISKIERRRSELQAAVTAAARDKKHVLEEQHALIEAEKNKVQGE
CEGLQYQVEVRNITQRIGSLSDQLDAASALSEPKENAFITFEFNHNNALSQLEEALNNLGRVRSSTTLPG
LCRARLKDPAIVKLQAAVIVETVDYHGHPRNVGGDLITAELTLADSIHSENQSSSIETEILDLENGTYEV
LFRPPSASRYILKLSVFERPIKDYPLFFDATEHNEPIKIYGRRGNGKDEFHQPVAVAVDDDGMIYILDTG
NSRIKVLNYDLEFQRHITNEGLEGRSCTGIGISQQGIVVVNWRTRKITEMSSLGDTIKSFSHNAFQEPID
VAVDRSYGHILVADNGQSCVFVFDSDGKILFQVGKRSTFKLITSVTVGPNGEIIVADSRIQVFSAKGDFS
EEIYSEGKGKGTYGGLAVDAEGRILGTRTDKGRSIIQVLKLGGGNILTEIDSHSSKLRRPSGIAVLPDNH
LVVVDLGNDCIKKYRYW

>Anopheles gambiae RNF223
MYSFSTSSKSGANSGGSSLVSSTTSSSGGSSTVSTSSIVGGEVAVTAASASVGATIQSMVASVVADPPEL
VAGAVERTPGAGCLPAVSSAAATSTALTSSAAVAILQQAPPASGAVLSASPRADQLVPKLGGPSPNNSSS
SVCSNGSSTSSGMFPEVFKLDDMLELENVGNGALSENGSTGGDFQLDNSSLSDLMEQDCTLCKNKFTSPR
VLSCLHVFCESCLDKLLAEGAGDKGLDGGNGGTIVCTICKQATPTGPKGAAGLHQDYILSNVLDLSTIEP
ALLACTSCKSKEMAISRCNDCANFLCASCEYAHQHMRCFEDHKVVQLADLRKSAEKVAIHKPLYCPVHSM
ENLKYYCFNCQTPVCNECLIGDHKGSEHNYHIISDAEKPMRQELECSMREAKSKIEYCNQATSKLESSLH
DLQSQYETARGLINESYQSCKAVLEQCRENALKNLEKLHSERELKIMDLYDNVAKSVEKIEVAAKYARKV
VDQANGPEMLSMKSMICAQLNQVMVTAPKVDVSFSIEFQSNYEKFEQLVPELFGKFRTESSLPVSVNETT
PSPTLPGVPIMVADAHHQPVNGGCGLSQGPLTASVTASSPISLPTSMQSSFDGDISTLGTTYMLPNVLTP
EPATGAANIAGPPNALSAGTPSPGVVVGTGPATTLPGLSSIAEYNLHRLANLAGETASNDLTDSIVPVVN
SNPATGPAGGATPNFTLADLISGNQHAIQALAKYSMGGQDMSNGAMLSAHPSLDSVPMMSDFPGVLPGST
SPILPVPPNMPNIDGPGMNGAGSDMAMSRFQPSMCVNGRTKATPMQIRTKFGSLGQTKGQFNSPHGFCLG
VDEEIVVADTNNHRIEIFEKNGTFKFSFGVPGKEEGQLFYPRKVAVMRTSAKFVVCDRGNERSRMQIFSK
NGHFIKKIAIRYIDIVAGLAVTNKGLIVAVDSVSPTVFIICEDGNLIHWFDCSDFMREPSDIAINGTDFF
VCDFKGHCVAVFSEEGTFKYRIGSEKITCFPNGIDISDAGDVLIGDSHGNRFHVACYSKDGQLQSEFECP
YVKVSRCCGLKITSEGYVVTLAKNNHHVLVLNTLYIQ

>Caenorhabditis elegans RNF223
MDPFDIDGLNVRLRDLQRQLQDDMEEFRDMAQMDDFAHPFGGIDAAINVDDPVDDANNDE
PAAGAENVLDREDNQPQPEAVATPDVPKAENEKEKEDEADDTILTSKGTEMKGLSCGICY
DPFNTGKRIPKVFPCGHTICLQCIKKLLNTRTFLGGNTVICPSCRQNTRYSLTTPADKIP
TNFCIQSLLEQRQQEKSTKEPEAVKCTECHTTVSAIEATTCSDETCQKEISEEELKNVDK
IKRPKETLICRSCIETNHSTHIFVSFSNVAAQYEANKKILLAESLLKKKLKAVDEALLAF
DKTKAEAIDHRRRMANALVNIRKEADHPDFYESLNRFMSSMKSSGELFKTLSSDLDKFEN
SSKARYHRSFGRDVLRPSAFPQETDGSKQEVKQAPAPVPVPPARPRPGPALNRERFRRLG
AVGGFVVPIVGRDANAPGGVRHLANEIDEFLAPNDQGLAAHFRRIFGDLPDDDIAIDVQI
AAAAPAGDAPAAGGGDDAVDGAGDFLGPAADLRLAGAAGAPLNIADRMYRRHRIAARAQL
AAPRRANRNGNNVRDLRMVRHHIFRAIAQNPGLHRNDAIQQAIDGLRDYIWNLEGPYARN
PFELDQIDMRRDLDNAQQRLNQLGARLQELEGNLRARDDPAQDAPWHDVVVQEPLNPLPP
IQGIEQEVADRAEIAELVNRIMGMVRNEAEAEANANGQRAPKNIEMPALLNPDANPDAPN
FKNLQPLQVQPYLRDLRALELEWIQKAQDRRARGGAQPPVEDVEQNPVVEGGTDAQRRAV
SPTQDEPAAKRNRQ


>Nematostella vectensis RNF223
MAQTYPNKTTSLERHALKISKKTNTKGYERKARPSQATPPIIKSKTLRVSKQSSLKKLQRALNDELRCSV
CYEVFSDPRTLTACLHSFCKECLHKMLSKRSKYIHCPLCRKKTAVPRRGVKGLPLNSVIRRLVDVHSSEG
MTSARHRKRHPGIK

>Amphimedon queenslandica RNF223
MAARANADISEILTCSVCFDDYKDPRTISCGHSFCLRCLESISDGKMVFECPVCRTETAVPEGGVSQYII
TFALKSLKDAVERTEGEEEYHFYCEKCRELLTYQTAATTHQGHKYHNIQVSYANHLEALKKSLICLRART
EDVAADREVLLQVESDILKQKEQIREEIVTVSDEIINAVRKATQQKLAQLDKIFDKKMETATQQRQESDE
MLKTFVGIQEEVEHKLSTSSCCQLLSKKKEISEQIHSAMTKEKNFKPKEKANGKFVRNSSLVSCSQNIGY
LFPKSSPRYSVKHDPVIMAGRPSTFTVSFSPLYPPYDSSPPIKCTLTSCDTRNATTTVCDVGKVQGFSHS
FEVVPIDRGEQLLTITTDDMLTAQKHDTFIAHPSPHMRGRPLKEIKNLKKPIGIAITKNGGLIVVNQGSN
SIEFINDTGRVSRDIKFTDPHGIALTDDEAYFVVTDKHSVRLIKKQSGNVIAIAGGEKKGYKRNLFNNPT
GVAINPMTKQIYVADTDNHRIVVLNEMALTRVTEFGKKGSSSGTFDEPMGMEFNTDKLYIVNNGSSCIDV
YSAIDHNPLFRIGASPSPLSYEGLSCPSSLTIDAFGYMYITEVTAPIFSFKSNAISRISIYKTDGQFVRS
FGGKGRTEESFYDPSGIAIDRRDGTLYVCDTDNNRIVLL

>Ciona intestinalis RNF225
MAGAKSLKNKLEDDLLTCPICCSLYNDPRVLKCQHSFCHGCLKGSMKAAKSNGKSINCAV
CRKPTPLAKKGLADLPKNYVIIGLSQMVKEHKESGKSPVVAKSSSGKNVGNVKQLVETFN
KKSIVEKPSKKRDKNFNIEVPKAGVVHDDENDDSSDLSTSASDSSGMTEFTPTDDSESTS
SGSDDSSSGSDDSDTDSESDSGSSDDEEPEHVPQRPTIKSSHGIKLGMKVFVFTAKGKPL
PGTVRVLEKYKRKPPNQTQVYAGVQMEKEYKKLTTEDILDMDDSYLFNWLMDFDQPCVAV
RIDQCCPAKVIQRFNL

>Ciona savignyi RNF225
MASASKSFKNKLEDDLLTCPICCSLYSDPRVLKCQHSFCRSCLERSVKASRTNCNSINCA
VCRKTTPLGRKRLQELPKNYVIIGLSQMVKDHKGNGGSSVDAEEPSMKENVVDVQQLVKV
FNKKSISSKKKSGKDKNDNKLIPNVMDGGDDSSDFSTSASDFSSEFTPTDSDQSGSSGSS
SGSSGSSGSSSASTESSSEDCDDSPEVLPPTPTMKSSLGIKIGMKVYVFSSRGKPHPGVV
RVLEKFKRKPPGQTRVYAGVQLEKENTRLTTDDIEDMDDSYLFNWLMDFDEPCTVVRVDQ
CCPAKIIESVMK

>Branchiostoma floridae RNF225
MATGGESPQGSLQIENFECSICLQVYMRPKVLSCGHTFCKECLVPLAKGSKSFKCPTCQATIRLERAGRA
GIEGLIDNISLSNLRDYVETSNRQGPRGSENPTVVSDESQCPKHHGEELRYYCPPCDAAICEECMFEDHN
THGVTRLRRTLQEQSIKAKEVCEEGTRLIETIRHKITELEQSRKRLAVEEGTLVKAVDQTARKLADDFAT
EVTKRANSLKDELHNLFAPSNSLVLDCKESLEIRLAQLVSSVESLEKSINQGEAIVVFSGRKMLKDLIEQ
SSQPTKAEQDVETGNIAVFTPTPYSFPAITLGKVQATSKIWERKKETDEEKVETSAAPKEDSPAPEVDGV
TNNNEAEENDEDIVEIEDKSGLKYKCREDQRNPLNSEGKKQYDRSFLLQFQPECIDKPDGLPKIVDVVLD
KPLIITPQQMQGRPQDFRGMGGSPGIPDPWPIPSYMKSPGGDHRRMEVFEKMQGILDKLTSQNFQTLCQQ
VLDLDIVTEERLKGVIDLVFEKAITEPNFSVAYANMCRCLIHLKVPMKDNPKQTVNFRKLLLNRCQGEFE
RDIDNKMEKDERMRKIELAKNEDEKKKMQAELEEEMANASRRSLGNIRFIGELFKLKILAENIMHDCVVK
LIKFNDEESLECLCRLLTMIGKDLDVERAKPLMDQYFEQLERNTKQKKTSSRLRFMIQDVLELRRVGIPQ
SLK

>Strongylocentrotus purpuratus RNF225
MADALKNAIAQSLECPVCLNTFTDPKILSCSHTYCKTCLDNLLDCHGNDQMLRCPVCRAETQVPNQEVSK
LPTNLALKSLIEDVKNHYQFCTNCKSEDKPQAVVYCQDCGKYFCSTCHNKHSQWPDFISHDVLAMSEIVS
GKVSVRRYRKCRKHPKDDEEWFCSDCRRFACFKCVVMEHTNEGHRVIEAAVYESNHMKSIEDLKSKADKK
RSCFQKYIDFIDEQKEHVSNVQKQCTDDINKAYEESVRQLTKKKEVLIGEVKGRTEGVEKELDEIKKSAQ
EHITHFTTIADVVTNRTKIPLDTDALAAHDTLCQDLQEALKQEDPDYKQSIQLSRKGKSVSFKRNVGMDE
LALGKIANAFAFKNIALPTKNSMNIMVGTPDGRMAVGCDTGGVEIFSADGQLQQTVLKGVKIRGVGFLSD
DRYVILDCSNNISLYRLQYTKLNVVFETLYNDEGEWAILNVGGDDQIYVSYWKAQKIQVFSPAGGKAIRE
IPCDGYEPQQITSYNDYLVTRSWDAIRLIDKEGVVKYELRELGYFPYATVSQGNTILIAKVNHDVGLVSI
DEYTNELKHIQNLVFDYKIEKPERWWYYLQQYRSGEIAFCTPDRLYIFHWK

>Anopheles gambiae RNF225
MSTNGWIHCNICFHSFERKERKFYHLSCRHVLCKLCMAKTNRGTMCPLCKKPLERFTELNNQMERKEKMY
YDPGSLKVFGVAYQSVIFQHKQREDMIRGILRCRSAVTQMKEMENAMRQKIVETQRRYEKFRTHRRNLQE
GLRQISPRYGSGPTVVQGAGCAGRTPLQPLTGNRASPQLALANRDGDPASRRYQDKGRPETVTFAASPES
SSADFSFGTPAPSNRSATVTDGSFTPAEQQHARLSTPQVSTERFFREGNKPAVTGQGDGVFADDSGISSM
QTPTSSLSFTGSSRMAQGMQQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQQQHQQLK
QQRQHPYTPSTPARHLPGTSSYRHSQIFPPTVPRP

>Caenorhabditis elegans RNF225
MSEKNTENPEILSTVLAGYCQCQVCYQPFNETTKLARSLHCGHTFCTECIRNVQNYGNSP
HLECPTCRAETKSNIENVAPNFSIMELARNFGLLGPPVDAPRTSSSCCSNKTNRDEMIDN
KFRELLVEVKSSLIHHFDVFRIKSQLWSLAEAEREVSNMAEALAGAVSECYYDSDDNSDA
NSEDQRVRTMLRFLAARDSDSETTEDSTDFLESNTTVPDRTLGGGFYRNFRRLFGDENEL
DDPGVVSGIEEAAAYRRFRGLFGNDNDSISQVSTTSGSFGNHENDHFCPFSDDDNISECS
DDYYPTLVMPETDEEETYKSFRCLFGDENGNLDFSDDEKEFSDNESSLSSVSTEDFPNSH
IEEAGRVLHRFLLLSALLNEE

>Nematostella vectensis RNF225
MATVRDEGYSPQSLNCRACHKVFTEPKILDCLHTFCQKCLGTHDILGAGTNSIVCPLCRKPTPIPESGVE
SLPSNFLLNNALDQLSVKSSKEYILHCTNCEDASDATQTLHRPSFCMQHKDEMFMFYCLECEGLICRECT
ILDHRGHKYAGLKDALEGRKILIDDLLGFCTVKKIPPVQDAVYEVKAMTAKLRARADAVKNDISSAADEC
VKRILARKQELLTQVDNMTQSKEGVLQAQQEQLEMELFRYTSSRDFVENVLLHGNEVEIIQLRQLMTNRL
NELNGVKLDYKEPEENDVIDYILDSEAVQKVAMTLGHVTTSQTFASLCIAKGLGVMSGKVGVESFFTIET
KDRFGNTTFEGDHCTPIQVIIQAPEGFFLGNHVANKRDGTLLVRYTPVTRGKHVIVVSIRGRQIEGSPFT
VKVVEGIDYKNVMAVFMKIGSQGTKSGEFRQPSSVATDTDGNIYVTDFVNCRVQKFDMFGKHLKDIGTKG
SKDGQLMNPCGVVVGSDGTLIVSDWDNHRVQMFSPEGKFVSKIGRKGSENGNFLHPSGLALNQDGDLVVI
DKDNNRVQVLKLDGSHVMSFGSLGNADGQLDCPSHVAVTPDNGYLVTDTGNNRIVKFDASGNHVMSFGTK
GSGEGRLDRPAGIAIDPEGFIIISDFQNHRVQVHSPEGEFYATFGSEGNIEGQFKFPSGIALTLDGHVIV
ADRHNHRIQVF

>Hydra magnipapillata RNF225
MAALSSSQVLENIHEQLVCAICLERFDDPKLLNCLHSFCCQCLHKIINQKPSDVIECPTCREVTKIPING
LKELKTNFFINSLLDLVHHKIEELKNEKKKGEQCEQCQEHDNATSRCIECDLVLCGGCIADHRRNRSTLD
HRIVVMEDDEDVNKQSLDKYTLTFCRYHTRNVIKYFCNTCDEAVCRVCTILEHREHQFVYPKDAIPQQKP
IIEELLEKTKENIPLLKATIKDIHSMSNTLRECRESLLLEINAKTQARIEALLLAKAKLLEELDRIHRGK
QKTLSLQSDAVELELGKISGCCAFAENVLKFGNEVEVLQIKSRLQQLNSITIKLDPEEDDSIQYVSESES
IEAVMESLGKICSSQTFASLSYAEGEGLETPRVGIESNIIVHAQNRHGKKKSGSDRVEAVMVLPDETKRT
LNALDNDDGTYTIRFTPDINGRHLLHITIKTKPINGSPFEIFVINRREYSEVGPSLLKFGQYGSKRREFK
SPFGVASDNEGFIYVADSYNHRIQTFGPRGEFVHMFGSHGDRKGEFNCPTDVAVSPNSKLVVCDNGNNRI
QVFGRNGNFIAKFGREGIKNGNFKSPWGVTVSPVSREIIVADTDNHRIQIFSSDGKFIMKFGCNGDRPGQ
FNSPCYVAANAENEHILVSDSKNHRIQIFDRRGKFIQLFGTQGQEDGQFNHPRGLSMDIANNIIVSDMGN
HRLQILTYAGKMVKCVGTEGNGDGQLSFPESVAVTPNGFIIVSDLSNNRIQVF

>Amphimedon queenslandica RNF225
MTLEEAPERDLFEELEENALTCAVCLGVYEDPRALDCHHVFCRSCLEHLPLVNQDGVNNLGCPTCRHLVP
LPPNGVAGFRVSFQTNNLKAFVEANRDRAVPVAVPRKQTCGSCTSENVSGFCTECRSTFCDGCTDLHLPT
CTKEATALNGASGTDKTNGHHQAPMPLDPAPLMQSDDWNVNTNTCVIHRQIADMFCENCEETICSRCVKR
FPHTEHESSCIPLAEALVRYRELLEDRLNPINKEIDFISTQLKPFSHRESEINEQGDTVKAQIHSRAEYL
IALIIESEEELSVKVDKVIKSKLHSLDERRESIEVTQEELKNLKDRIELCLKTGNQEDLLKTKQWIPKIN
HVMFNIDATKLKPSEVADLSFVPDEVSDSLTRLGNLSSTFEGKDPSCDVQILEQHLFIDKSPDVTLVLSV
KYTNLPFVIIPLSSMKCIIKSSNRSFIKAIISLGDAAHQYVVKFRPTSNGLYKLSLKIDDIETEDQAVLI
PFNPMYLPNITPTTVMKYIRKPIGLAVAQDKRIIVLNDFNEVVILDMHGRNIRTLSRPNNGSRFTSTRHA
AVTSDGCFLMTDQYSILKVTMDGQLLKTIGSKGNGRLQFNTPFGITIDEKTGKVYVADLLNHRIQVLNRD
YSFSHSFGSRGKALGQFEQPNGTALDSQGNVFVSDTFNHRIQKFSQSGEYISYFGSEGPNPGQLKLPEKI
VINNDYIYVTEHLNARVSVFTTEGNFVRCFGKQSIEGGLVWPRGLTFDEDGSLYVCDFRSHCIFKY