>Homo sapiens RNF223
MSSGQQVWHTAVPPPRRSSSIASMPRSPSSAGSPRSPGTPGSERVASPLECSICFSGYDN
IFKTPKELSCTHVFCLECLARLAAAQPVGRPGGEAVPCPFCRQPTAVPPAGAPALCTSRQ
LQARMPAHLRREEPVWLEGTKLCCQPLPTTPGREPGFVCVDVGLSKPAEPPAPARDPAPR
RGRLARCWARCRDWRRMALVSALLLMLFCVALWPVQCALKTGNLRCLPLPPRPPATSTAA
SPLGPLTDN

>Gorilla gorilla RNF223
MSSGQQVWHTAVPPPRRSSSTASMPRSPSSAGSPRSPGTPGSERVASPLECSICFSGYDN
IFKTPKELSCTHVFCLECLARLAAAQPVGRPSGEAVPCPFCRQPTAVAPTPGREPGFVCV
DVGLSKPAEPPAPARDPAPRRGRLARCWARCRDWRRMALVSALLLVLFCVALWPVQCALK
TGNLRCLPLPPRPPATTAAASPLGPLTDN

>Macaca mulatta RNF223
LSHSSETCRPASRCGTRPCPPIAGAAPQPRCPGPPARLAAPGPTPGSEKVASPLECSICF
SGYDNIFKTPKELSCTHVFCLECLARLAAAQPVGRPGGEAVPCPFCRQPTTVPPAGAPAL
RTSRQLQARMPAHLRREEPVWLEGTKLCCQPLPTTPGHKPSFVCVDVGLSKPAEPPAPAQ
DPAPRRGRLARCWMRCRDWRRMALVSALLLVLFCVALWPVQCAIKTGNLRCLPRQPPSPS
STGGSPTGGLKGAAATG

>Canis familiaris RNF223
YQSGVQAVQTALPDSRCGTAMSAPSRTSPTAPAPRSPSTPGSEKVASPLECSICFSGYDN
IFKTPKELSCTHVFCLECLARLAAAQPTGRPGGEAVPCPFCRQPTAVPAAGAPALRTSHQ
LQARMPAHLRREEPVWLEGTKLCCCLPPSVPGLGEPGIVCVDVGLSKPAEPTAPTPAPGP
ARRQGRLARCWARCRDWRRVALVTALLLVLFCVVLWPVQCALKTGNLHCLPRPSTTITTA
TAFSSRPLADN

>Felis catus RNF223
APGRTSPAATVPGSPSSASSPRSPSTPSSEKAASPLECSICFSGYDNLFKTPKELSCTHV
FCLECLARLAAAQPTGQPGSEAVPCPFCR

>Bos taurus RNF223
MSSGPQVWHTTMPLTSRSSPTATVPRSPSSASSPRSPGTPGSEKVASPLECSICFSGYDN
IFKTPKELSCTHVFCLECLARLAAAQPTGQPGSEAVPCPFCRQPTAVPAAGAPALRTSRQ
LQARMPAHLQQEEPVWLEGTKLCYYPSPSVPGPVEPGFMCVDIGPSKPPEPAVPVPTPDP
AHRRGPLARCWARCRDWRRVALITALLLVLFCVVLWPVQCALKTGSLRCLPRPPSTAATT
SSLGSLAYN

>Equus caballus RNF223
LSHSSEPCHQASRCGTQPRHATAGAVPQPQCPGPPAQPAVPGPPSTPGSEKVASPLECSI
CFSGYDNIFKTPKELCTHVFCLECLARLAAAQPMGQPGGEAVPCPFCRQPTAVPAAGAPL
RTSRQLQARMPAHLQHEEPVWLEGTRLCCCPPPAAPGLTAPGFMCVDVGLRKPAEPAAPP
PTLGPTRPQGRLAHCWARCRAWRRVALVTALLLLLFCVVLWPVQCALKTGNLRCLPRAPT
TAATAAAFSLGPLADN

>Loxodonta africana RNF223
LCLSHSSEPCHQTSRYGTQPCHPTAGAAPQPQCPGAPTQPTPGTPVSEKVASPLECSICF
SGYDNIFKTPKELSCTHVFCLECLARLTAAQPIGRPGSEGVPCPFCRQPTAVPPDGAPAL
RTSRQLLAKMPAHLRREEHVWLEGTKLCCRPPPSSPGHNSPTFICVDVGLSKPAEPAPPL
FPPGPLRRQGRLARCWARCRDWRRTALVSVLLVVLFCVVLWPVQCALRTGNLQCFPRPSG
AATTATSKMLPLGPLADN

>Myotis lucifugus RNF223
QQCAGSKTAGPPCQWSRMASEATNIPSPVVRQIDKQFLICSICLERYKNPKVLPCLHTFC
ERCLQNYIPAHSLTLSCPVCRQTSILPEKGVAALQNNFFITNLMDVLQRTPGSNVEESSI
LETVTAVAAGKPLSCPNHDGNVMDFYCQSCETAMCRECTEGEHAEHPTVPLKDVVEQHKA
SLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDELQKTLNVRKSVLL
MELEVNYGLKHKVLQSQLDTLLEGQESIKSCSNFTAQALNHGTETEVLLVKKQMSEKLNE
LADQDFPLHPRENDQLDFIVETEGLKKSIHNLGTILTTNAVASETVATGEGLRQTIIGQP
MSVTITTKDKDGELCKTGNAYLTAELSTPDGSVADGEILDNKNGTYEFLYTVQKEGDFTL
SLRLYDQHIRGSPFKLKVIRSADVSPTTEGVKKRVKSPGSGHVKQKAVKRPASMYSTGKR
KENPIEDDLIFRVGTKGRNKGEFTNLQGVAASTSGKILIADSNNQCVQIFSNDGQFKSRF
GIRGRSPGQLQRPTGVAVHPSGDIIIADYDNKWVSIFSSDGKFKTKIGSGKLMGPKGVSV
DRNGHIIVVDNKACCVFIFQPNGKIVTRFGSRGNGDRQFAGPHFAAVNSNNEIIVTDFHN
HSVKVFNQEGEFMLKFGSNGEGNGQFNAPTGVAVDSNGNIIVADWGNSRIQVFDGSGSFL
SYINTSADPLYGPQGLALTSDGHVVVADSGNHCFKVYRYLQ

>Gallus gallus RNF223
MSCFTQLWHSSTPDPIVITSPVDSKRKARSPTDKPGSPNPTSPKPASPVECSICFNTYDN
TFKTPKLLQCSHVFCLECVARLSKGLPPNHPEDQLPCPFCRQLTSIPQEGAPALETSKEL
LATLPPELQQEKVLWMEGTKLCCRQASDDPENPDSCISIDVAMSKPESPEVPPTGLVGRL
SRCDMCDDWKRIVLLSALIIILFCIILWPVQCALKTGNLRCFTRPHGPQRPFCAHTWMGA
LTSS

>Pelodiscus sinensis RNF223
MSWLPQMRRTETPRSPSSPIPAAQDEVPIPISPIIVTSPTYGRKLSSPTSPKPTSPIECS
ICFNTYDNSFKTPKLLQCTHVFCLECVARLTSALPPHRVEDHLPCPLCRQPTEIPLEGPP
ALQTSKELLSTLPPELQREKLVWMEGSKLCCRQSSQDTEDPNACIWLDVAMSKPNVPTTP
TEGMAGRLSRCAICDDWKRIVLLSALAIILLCIILWPVQCALKTGNLRCFTRTVTYTRPG
TTLFTQNNSTVPGTSLLSN

>Xenopus tropicalis RNF223
SRLECSICFTKYDNIFKTPKLLQCSHTLCLECVARLVAALPPEKQAEDVLCPFCRQPTHI
PDDGAPALQTSQELLSTLPAHLQHEEPVWIEGNKLCYKKPPETNPDQHDLCICVDIGLSK
KDSPTEPRQRRGCFRGCSDWKRLLLIAFIVAVLFCILLWPVECILKTGSLRCTSEPVPTR
IPVVPAVVTHGPIDER

>Takifugu rubripes RNF223 b
METLEEDLTCSVCYSLFSDPRVLPCSHTFCKTCLDNLLQVSNNYSIWRPLRLPLKCPNCR
SMVELPPTGVEALPSNVSLRAIIEKYQSDNEPRPPSCQEHHRQPLNMYCIQDRQLICGLC
LTVGQHQGHPIDDLQAAFTREKRTPSLLLAKLSESRWAQVVCELGEQLEQEKARCEDLLR
QDRHNVSQYFHSLEVVLAKKRRACLDALDKAAGEVCRAYDPLIHKVKELQEEQLDLVSFG
SLVEEEESPLVFLEKVHLFRERVEEFTACPLPSVVNMSLTPRAADFLQQHWASVSIQGLE
DAPVPKLAVSFQRRACTWISLQREGVQTVHQLQLCITTQGEHLVVLLQFVGDHVEARHEL
IETL

>Gasterosteus aculeatus RNF223 a
MFPAPRSAAAALFHLTTPPDVPPPQQVPDSLPPPTHPSISNMSEDREGRAAAPPPNVKPK
LGQTERRSKEGTWKKEKPPKERRSRSTHSEREDERRGRERDRHRGGRSRRGRSEEARGRD
GRGSASGRVKPPEDEVEDTECAVCFCSYDNIFRTPKLLACGHTFCLECLARINVTSARLK
TLSCPVCREPTELPHGRDLPRLGTNKDVLGRLPPDMRRVLSIRFKRSKGKLQLKNPPPAG
RDKNSVRQPSKIQGGAVPAGGHSDLSALE

>Gasterosteus aculeatus RNF223 b
IELQSPEVDAPSADELECKICYSAYNLAARRPKVLGCCHRLCAKCLAKILDLGESPPDAV
ACPFCRYVTGLPGQAVSGLPDDCNLAAALALQSRNQRNLHSRQEATTELLLSPGLLSSLM
GGGPPTTGSPNFVVITIMETPPPPMPLPASGEDLRRQPRGPRGGFRSSSLDSTASIAHRW
TAWNCASQLCQTSARVLVWVLGLLYFSSLPMGVYLLIMQRTTVGLLLVSLVPASLVVIMV
YGFCQCVYHELWDCVPP

>Oryzias latipes RNF223 b
MDSLEEDLTCSVCYQLFSDPRVLPCSHTFCQPCLENLLQVSTNYSIWRPLRLPIKCPNCR
SVVELPPAGVDALPSNVSLRAIIEKYQRDEEPRPPSCQEHPRQPLNMYCIQDRQLICGLC
LTVGQHHGHPIDDLQAAFAREKATPQQLAELSEHRWAEVCELGEQLEQEKTRCEGLLRQD
RDDVTQFFHSLALVMAKKKEAYLEVLDKAAAEVSQAYDPLIQRVKELQEEQLELMSLGSS
VEEEEESPLAFLEKVHLFRERVDKFLKAPLPHAIKLSVTPRAADYLQQHWPPLTIGALEE
APVPAVCCCTRRGSASWKRSPVYSVAWGPDSDRILYTSGVQLTIKPLQPSAKVMQWKAHD
GLVLKVDWNSVNSLILSGGEDCKYKVWDSFGRLLHSSSPHDYPVTSLSWAPDGEMFAVGS
FNTLRLCDKTGWSYAVEKPHTGSLFSLAWSADSTQLAGACGNGHVIFAHVLEQCWEWKNF
VITLTKRRSMQVTNVMNDAVDILDFRDPVIKASLAFAHLVVATSLQCYVYSSRNWNTPLI
FDLKEGTVSLILQAEKHFLLVDGAGLYVFSYEGRLISSPKFLDMRADSLNAQVISLCDDT
IAIRDNRDEKVILFFDVLTGKAVGDGNPLTHKLEVVEIALDQCGPATDRKIALIDKNRDL
YLASVRHFGRETKICKIGTMVDNIAWNDAANILCGIQDNQLTVWCYPSVVFTDKELLPKT
LHIRDSSEFNSAPRILNFSGTKVTLRQGDGSLVYSSVPPYIALLHEYCSSARWEDALCLC
RLVKDQSLWACLASMAMANRELTTAEMAYGAIGELPRVEYISMIKEQPSKESALAHMLLF
SGQVQEAESALLQAGLIYQAIQVNIDLFNWERALELAVKHKTHVDTVLAHREKFLQKFGK
KETNKRFLHYSEGVDVNWENIRAKIEMELSKEREKAANASVRSNGASY>Ciona intestinalis RNF183
MAMKYKSSDSRKVPAQPPQWNQFLICSVCENEFNRTDRCPISLGCGHTVCRGCLGDLKHP
QCQFDQNSITCDISDLPVNSALLLLVPEEESHKGSVEMRGVSQKGKENFHPGNIAQCVKL
YDKSKKHIEELALLLRPNKGNELSRPMQRKLVALINCQLVEEEGRKRALRAGRALGERSA
TELILLHQNPTTLSASLWAAVRARGCQFLGPAMQE

>Ciona savignyi RNF183
MENRRTHIEASDVDLALEEAGHCGLYHAWLILLMAVPNILVGAHMGSSVFEAMIPTHYCV
TQQADDITTACNITWNPTNDALAVPEDLDGKHSPCHMYYYDNVTDIDCKFWTLDKNSTPT
VEPGIRTCDHWIFD

>Branchiostoma floridae RNF183
MELQCGLCGQQYSRPRVLSCLHTFCLACLESLMKRQDREGEVCCPVCQQRTLLPSSDPSTLPSDPIIDAL
LEVKSVEAGAVTCTSCEENSSAVCRCLDCAAFLCRNCQGAHRFMKCFKSHRLVELENLSISDLASPLKLL
TCTKHTGEFLEFYCNTCDALVCLKCTAIDHRRTSHQYVYVKDAAAVMKERMLYLLENGRKRSEEVRVQLL
RMEEKIDHVEVEKMKVKGEVDKVFHTLLSKLEDRQEEMCKEVETLYLRQKQFLNKQGKSLQLDEERLQAG
TRFVEKLMTYGSTSDLLTCGKLVTSQFDLLFKQCPGNDMKSSGAVGVSVDYHAFGNLLSRIQIQIKTVPK
IGVSSPVTQAGCSAVGESHRVESGSAEATPPSPKEQFSPSSSRSNLSEERKGSDSAQMDVTELHKVRSFG
KRENAGNCPYLTCVAVGANGNIYALDMYKSVVQVFDKEGKYCFNIGGRFQAPGRLEKPVELATSSKGYIA
VRESNGRMQIYSDTGQFIFKFQLLRVQRPRSMAIDRKGRFLVGDVGTDRCPHGVVIYSSDGKLLRKITKL
SAYTSSLAMSAQNHLLVVDHRAHCIQVYSEEGNHLRTIYPRGSRIYMRGIIVNQNKIILVTSEKVRNNMY
CGGLVVMAKDGTVLDKFKFPEKQPVVHVALTQDGCPLAILQGNSDICLYGSIYSK

>Drosophila melanogaster RNF183
MNNSPKDPYRGGGGGSGPQDGAGSTGVSINIESDDNDSTTAEHDYLLPASSSSVTSGGGV
GGGGVVSGGSTSTSVNLDHQHVPTARSSSLSGGNVRHGNILSTFLARQRSYTPASNSSSP
VRVTTQMNRRLQQSRSMGANTGGSLEEPSSAGAAGGPGASPGTAAGVRQMGFWRVNLNDV
FSLSTASSTEALRSFISHSNFRYPPATTAAPAAPGPQPAANPVDNSHILLGQQQPSMPEP
VSPLRYGRVSAVSGASSAGAMGRSISLREGEMRHNHSLNGGRHNASAIGLNSNPAAFDER
EANESQDLDESDGNVENGGGGGNPPEPAENNPEDEHLISDDMVVQILSHFVRYLPLIFIL
FFKFLHDHLLGIVDLLVLQTVMYNVNRSVRNQVARLAQKNYAVMVRDTFLVAVVVTVRLF
LATSPPDPFGLIVPPSRKSVFIEVTALPFHTSSEGDSPTTSSEPIKTNMYKDTYKSLNVI
PLGMLLYYIAVSDLIIKLLTMLVKLIITMLPHHLMRLKVRARLYVLVEYISQFYRAMTPI
TQWLLFLYESYSGLEVVSGGLFSAMYLGAKIFELVERGKSLKKAIVTFRKNIDSERPPTK
DELDAAGALCPICHDAFNTPTVLECGHIFCDECVQTWFKREQTCPMCRAKVSDDPAWQDG
STTFFHQLY

>Amphimedon queenslandica RNF183
MDSSQKPVVASVPPRPSNEVVNEASSSSLLCPRCNDSYTDPRLLSCLHSFCTPCLQSLVDGKASKQTVTC
PTCREITKLPKGDGGITSLPCNLHLISNQEDILLKITSSSTSPSCNSCDDGAGCAVAYCTDCMDFLCQTC
WGAHQTVKVTRSHSSFTLEKARAMPRGDLLKLLPSSSLSCHDHPRQELDFYCNQCSVPVCVKCTIISHRD
HTVTEAKPQAVKTKEELTKVMTAVHESEEKLKAVLDAGERTNEMIRKQKDEVDTLIRQSIASLQKLLEGR
EQSLIEKSNDVSLAKQTRLSRQLDTFKSLMEDMKACQDLSQTAVRDYGDVELLSIAHTLQARATELQEKF
SGTSLTLCESPSVNIEMNVSKIASGIASLGTVNGVDPICPEETTAEIPSCIHTVGKELQIKVISRDSTGK
RLNKGGARVTGQIACKGGGGGKKHVSATIIDSGDGTYTASFIPQQFGQQKITLSLNDGPIKNSPFNLYIS
RDYASISSLKGPLVTISDIDSPFFIAIADNGDVFVTSHFHHCVYVYSSNGDYKRVIGSKGKGQLQFLQPA
GIAISGNTVYVAENNGNRIQRFTRKGEFIDTFGSEGSGKGQMKYPWGICLDSNGKLYVTEYGNNRVQVFN
KDGSHSHFINATSNKMDRRFDTPEGVAIDASGRLHVTGFNSNNVAVFTLDGTFINKYPVKQSPCGIAFDP
VGNPLLSMNGGTSKIGVFDSSYNLIASFDKVFNVRGIAVANDGTIWVAEIGHSRLLNF

>Ciona intestinalis RNF223
MSPIRTRASSTPDYNIFKENIRFAGHMMASSIDLLTKINDEFLTCQICFETYTRPKSLNC
QHTFCLKCLEEYTPPNSVRVICPTCRSEQPLTADGINGLKDNFFISSMSDMLKTVKEIRS
EDKDGTSLMCDTCDHDNRKVAIARCLDCTDFLCNECSTWHIRTKLTRRHKIVSLSEFESG
IHNQELKSRAKIYCMIHDGEAAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQ
IENLLKTAKEKTDVLRTLQRTLKDIKQTKKLDRDKAESEIERICEIFINTIHRRKQELTD
ELHNMHATEVKRINADEDSVEMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQL
LETHPAATYHNTNQTDNIQFDTNADASVRVIRDNLGRLIIQKATFDEVVNKVLKQLDSFC
VADNHSMVRRNGKTLIQRAESTETKLVVQLGKQGIADGEFESTPNLAINSVNEVITADYD
GAKIQIFDPQGNFKDSFVTEVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGF
GKGQFGNCSSIAVNSENRIVVADVGKHCISVFTDTGKMLLQFGAQGKGESKLVEPRYVAC
DSQNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGLCTDEHDNILVADCWN
HRVDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLIISPTTLIPDFDQLAGQFDLPNL
LSNKVSWWLTSPNAQDPEHAVNGLTNGNIHNTIATSSITRKPSKTLASKKSSSSDSDLTA
MTPLSPIAPPSTPSLTGQQALDAVLNGTNGGSNGSLSSTGSGKIHYQGKVTSLNDHVPVT
MVNGT

>Ciona savignyi RNF223
MMASSIDLLTKINDEFLTCQICFETYTRPKSLNCQHTFCLKCLEEYTPPNSVSVMCPTCR
SEQPLTEDGINGLKDNFFISSMSDMLKTVKEIRSEDKDGTSLLRVGRLMLSESCGIHDGE
AAKIYCQSCQCPICHECVESGHSRHRRCSLKEEVLNQSHQIENLLKTAKGKADVLRTLQK
SLKDIKQSKKRDRDNGEAEIERICEIFISTIHRRKQELTDELHNMHAAEVKRINADEDSV
EMQLSSLVSCTEFSRKVLEYGTPKEVLGLNNQMTQILHQLLETHPATTYHNTDQTDNIQF
DTNADASVRVIRDNLGRLTIQKATFDESINKVGWGDVINVGKLYTWFVLARRNGRTILQR
VESTETKLVVQVYQWEWAPMSTSIRVYTGKVGGSPLTLLMAINIMDIISTQVIVPSVVKT
TDVRFSKVKTINIWSRFMLLFCVQIGKQGIGDGEFESTPNLAVNSVNEIITSDYDGAKIQ
IFDPQGNFKDSFFVYNPQVNKRMCKPAGIAILDNDDIVVCCEDQVHIWTHEGKSVLGFGK
GQFGNCSSICVNSENRIVVADVGKHCISVFTDTGKLLLQFGAQGKGESKLVEPRYVACDS
QNNIIVSDGGDCSVKKFSSQGEFLLSFGAEGPERGQFQGPRGICTDENDNILVADCWNHR
VDIFTPDGCFMRHIATGADSLHFPWCISLTTNGKLVLSEDYSWSVKVFTLPEYLHESTIS
SLDEIISPTTLIPDFDQLAGQFDLPNLLSNKVSWWLTSPNSQDPDSAVHAMTNGNIHNTI
ATTSVTRKPSKPLTSKKSSSSDSDLTAMTPISPVSPSTPSLSSQQDALTNGAITGSNGSL
NSAGSGKMNFPGKVTTLNDHMPVAIVNGV

>Branchiostoma floridae RNF223
MASSLLSQISEDFLKCQICHDTFKRPKVLSCLHTFCLQCLKSYLKKNPRVSIKTFLCPLCRQETATPPTG
ADGLKDNFFVSNLLEALSTLKTFKVKGFVACGGCSRSASSRCMECEDFLCVHCHGVHAKLKVTRDHTVLS
IRELSSEGGSAQIRAYTKATLCPVHEGETMRFYCETCQKPICRDCTVLDHSKPDHKYVYFKDALSSRKDE
IQDLLTSCRGMVEGLEETKERLESEEGELRETKEGTLKDIEEWVAGLVLDTQRKKEELKDQVETIYNEQL
KELSAAKSRVEMTLNDIDSGCGFSEKVLDIGTDFEILSMRNEMAARLSDLTKMERPKVDQNRSGLIKFRP
YGGIGELVKLGVGELDSRTVGELVVKFGGWGPQQGQFKHPGGVAVSSAGHIIVADTGNHRIQVFDSRGVF
LRAFGFYGSADDAFSHPHDVAMTTDDRILVTDKGNKLVKLFTLEGKLIGKIGTGNLKEPTGVAVYKHGGV
AVTDTRDVKTFTRTGVMSATCTSDDPGYSHYLCTDDEGRCSKCSL

>Strongylocentrotus purpuratus RNF223
MAAKAVEKLVRELVCAVCMNTLKNPKSLSCRHTFCEECLKQCDEASQGRSGGIRCPMCREVTTLPRVGVA
GLKSNPLIYRVLEVLEAKKNSTQIICDACGDSESPLTTYCRDCLHFICDSCASSHSKMKLLMKDHRTVPL
DKIENGTIKIKWMEEGLWRKAHECEEHGGELRRFYCRTCKKRICRDCIVLDHTKPDHDCTTVQKMFQETK
EKLQDRLKTCEEKEEHVSTILNDVDARETGANQKFHKMKKSIRDQKSKAVDNLDKAEEELLQKVDVLEEK
LNVKLQAFRDNMNTTKENLTRAQSKAKEVVSAKEVTDISRGPFLPEDVLKELEELIASDVTMTISVDLVA
LKSTMIEGSKMPEQMARFAKLSLSRVWRETLMFPIPDDKDIVNIFSDGKAMYVACKKVIYKKLLSSEAKP
SIWLNLQLNASIKQVVDVAISMKDQTFSFIADTDAACTQLYRLKLEDFPKSDEHTTVPPCPLNPSARLTV
DSQSLIVVADNKPAISSSSQTTSSESEPVSETKQKKPTSSPVVEGSQTVSAVGSAKSLSKAEGTLSPSDS
KGKETRPEIPKAGSDIFTISTKIYRENTEKSTSQLSSDNFEVPLDRVRSLAAAKTGQLVFLRGDKKAVII
TDKDGGVQLEIKEPGRMYLSISCTESDALYVLSAFWGSHIVTLAKHSLEDGGPLEYIIDELDVSADFQSD
EWPVIGNYGNDLVIMHTGDGIRVFSAEDWDILDDKDANGQDQDDDKEEDV

>Apis mellifera RNF223
MEHNQATDINEIPKIVGGSVAKDGQYPYQASLRYRNRHFCGGSVLNERWILTAAHCLSSFNDTAITVVLG
TNTLDKGGDDYQSEKIIGHPRYSSILIRNDIGLIKLNKNIVFGDKVKPIQLPSENFNKIDYPAVLSGWGT
TSYPGQVPNELYYIQLNVIDQKQCLNVSFRVTNDNICTLNKRGEGACHGDSGGPLVADNVQIGVVSWGTP
CARGQPDVFTRVYSYIDWINIFYRTRLGERMVSMSSMLVETVSINYEDFNESFLTCGTCLCVYDGGEHTP
KLLPCSHTVCLHCLTRIAASQTRETGAFRCPICRELITIPRGGVPALPPSFLVNQLLDLMSRQRREVIPK
CSVHINQELLFCETCDTVFCTVCTGGNHAGTSPGCTEHTIIPFSIAIKRMSEILLYKANECISKLTQAQD
SVSTELQRLEASTEKCLNAVDTEFAEIISKIERRRSELQAAVTAAARDKKHVLEEQHALIEAEKNKVQGE
CEGLQYQVEVRNITQRIGSLSDQLDAASALSEPKENAFITFEFNHNNALSQLEEALNNLGRVRSSTTLPG
LCRARLKDPAIVKLQAAVIVETVDYHGHPRNVGGDLITAELTLADSIHSENQSSSIETEILDLENGTYEV
LFRPPSASRYILKLSVFERPIKDYPLFFDATEHNEPIKIYGRRGNGKDEFHQPVAVAVDDDGMIYILDTG
NSRIKVLNYDLEFQRHITNEGLEGRSCTGIGISQQGIVVVNWRTRKITEMSSLGDTIKSFSHNAFQEPID
VAVDRSYGHILVADNGQSCVFVFDSDGKILFQVGKRSTFKLITSVTVGPNGEIIVADSRIQVFSAKGDFS
EEIYSEGKGKGTYGGLAVDAEGRILGTRTDKGRSIIQVLKLGGGNILTEIDSHSSKLRRPSGIAVLPDNH
LVVVDLGNDCIKKYRYW

>Anopheles gambiae RNF223
MYSFSTSSKSGANSGGSSLVSSTTSSSGGSSTVSTSSIVGGEVAVTAASASVGATIQSMVASVVADPPEL
VAGAVERTPGAGCLPAVSSAAATSTALTSSAAVAILQQAPPASGAVLSASPRADQLVPKLGGPSPNNSSS
SVCSNGSSTSSGMFPEVFKLDDMLELENVGNGALSENGSTGGDFQLDNSSLSDLMEQDCTLCKNKFTSPR
VLSCLHVFCESCLDKLLAEGAGDKGLDGGNGGTIVCTICKQATPTGPKGAAGLHQDYILSNVLDLSTIEP
ALLACTSCKSKEMAISRCNDCANFLCASCEYAHQHMRCFEDHKVVQLADLRKSAEKVAIHKPLYCPVHSM
ENLKYYCFNCQTPVCNECLIGDHKGSEHNYHIISDAEKPMRQELECSMREAKSKIEYCNQATSKLESSLH
DLQSQYETARGLINESYQSCKAVLEQCRENALKNLEKLHSERELKIMDLYDNVAKSVEKIEVAAKYARKV
VDQANGPEMLSMKSMICAQLNQVMVTAPKVDVSFSIEFQSNYEKFEQLVPELFGKFRTESSLPVSVNETT
PSPTLPGVPIMVADAHHQPVNGGCGLSQGPLTASVTASSPISLPTSMQSSFDGDISTLGTTYMLPNVLTP
EPATGAANIAGPPNALSAGTPSPGVVVGTGPATTLPGLSSIAEYNLHRLANLAGETASNDLTDSIVPVVN
SNPATGPAGGATPNFTLADLISGNQHAIQALAKYSMGGQDMSNGAMLSAHPSLDSVPMMSDFPGVLPGST
SPILPVPPNMPNIDGPGMNGAGSDMAMSRFQPSMCVNGRTKATPMQIRTKFGSLGQTKGQFNSPHGFCLG
VDEEIVVADTNNHRIEIFEKNGTFKFSFGVPGKEEGQLFYPRKVAVMRTSAKFVVCDRGNERSRMQIFSK
NGHFIKKIAIRYIDIVAGLAVTNKGLIVAVDSVSPTVFIICEDGNLIHWFDCSDFMREPSDIAINGTDFF
VCDFKGHCVAVFSEEGTFKYRIGSEKITCFPNGIDISDAGDVLIGDSHGNRFHVACYSKDGQLQSEFECP
YVKVSRCCGLKITSEGYVVTLAKNNHHVLVLNTLYIQ

>Caenorhabditis elegans RNF223
MDPFDIDGLNVRLRDLQRQLQDDMEEFRDMAQMDDFAHPFGGIDAAINVDDPVDDANNDE
PAAGAENVLDREDNQPQPEAVATPDVPKAENEKEKEDEADDTILTSKGTEMKGLSCGICY
DPFNTGKRIPKVFPCGHTICLQCIKKLLNTRTFLGGNTVICPSCRQNTRYSLTTPADKIP
TNFCIQSLLEQRQQEKSTKEPEAVKCTECHTTVSAIEATTCSDETCQKEISEEELKNVDK
IKRPKETLICRSCIETNHSTHIFVSFSNVAAQYEANKKILLAESLLKKKLKAVDEALLAF
DKTKAEAIDHRRRMANALVNIRKEADHPDFYESLNRFMSSMKSSGELFKTLSSDLDKFEN
SSKARYHRSFGRDVLRPSAFPQETDGSKQEVKQAPAPVPVPPARPRPGPALNRERFRRLG
AVGGFVVPIVGRDANAPGGVRHLANEIDEFLAPNDQGLAAHFRRIFGDLPDDDIAIDVQI
AAAAPAGDAPAAGGGDDAVDGAGDFLGPAADLRLAGAAGAPLNIADRMYRRHRIAARAQL
AAPRRANRNGNNVRDLRMVRHHIFRAIAQNPGLHRNDAIQQAIDGLRDYIWNLEGPYARN
PFELDQIDMRRDLDNAQQRLNQLGARLQELEGNLRARDDPAQDAPWHDVVVQEPLNPLPP
IQGIEQEVADRAEIAELVNRIMGMVRNEAEAEANANGQRAPKNIEMPALLNPDANPDAPN
FKNLQPLQVQPYLRDLRALELEWIQKAQDRRARGGAQPPVEDVEQNPVVEGGTDAQRRAV
SPTQDEPAAKRNRQ


>Nematostella vectensis RNF223
MAQTYPNKTTSLERHALKISKKTNTKGYERKARPSQATPPIIKSKTLRVSKQSSLKKLQRALNDELRCSV
CYEVFSDPRTLTACLHSFCKECLHKMLSKRSKYIHCPLCRKKTAVPRRGVKGLPLNSVIRRLVDVHSSEG
MTSARHRKRHPGIK

>Amphimedon queenslandica RNF223
MAARANADISEILTCSVCFDDYKDPRTISCGHSFCLRCLESISDGKMVFECPVCRTETAVPEGGVSQYII
TFALKSLKDAVERTEGEEEYHFYCEKCRELLTYQTAATTHQGHKYHNIQVSYANHLEALKKSLICLRART
EDVAADREVLLQVESDILKQKEQIREEIVTVSDEIINAVRKATQQKLAQLDKIFDKKMETATQQRQESDE
MLKTFVGIQEEVEHKLSTSSCCQLLSKKKEISEQIHSAMTKEKNFKPKEKANGKFVRNSSLVSCSQNIGY
LFPKSSPRYSVKHDPVIMAGRPSTFTVSFSPLYPPYDSSPPIKCTLTSCDTRNATTTVCDVGKVQGFSHS
FEVVPIDRGEQLLTITTDDMLTAQKHDTFIAHPSPHMRGRPLKEIKNLKKPIGIAITKNGGLIVVNQGSN
SIEFINDTGRVSRDIKFTDPHGIALTDDEAYFVVTDKHSVRLIKKQSGNVIAIAGGEKKGYKRNLFNNPT
GVAINPMTKQIYVADTDNHRIVVLNEMALTRVTEFGKKGSSSGTFDEPMGMEFNTDKLYIVNNGSSCIDV
YSAIDHNPLFRIGASPSPLSYEGLSCPSSLTIDAFGYMYITEVTAPIFSFKSNAISRISIYKTDGQFVRS
FGGKGRTEESFYDPSGIAIDRRDGTLYVCDTDNNRIVLL

>Ciona intestinalis RNF225
MAGAKSLKNKLEDDLLTCPICCSLYNDPRVLKCQHSFCHGCLKGSMKAAKSNGKSINCAV
CRKPTPLAKKGLADLPKNYVIIGLSQMVKEHKESGKSPVVAKSSSGKNVGNVKQLVETFN
KKSIVEKPSKKRDKNFNIEVPKAGVVHDDENDDSSDLSTSASDSSGMTEFTPTDDSESTS
SGSDDSSSGSDDSDTDSESDSGSSDDEEPEHVPQRPTIKSSHGIKLGMKVFVFTAKGKPL
PGTVRVLEKYKRKPPNQTQVYAGVQMEKEYKKLTTEDILDMDDSYLFNWLMDFDQPCVAV
RIDQCCPAKVIQRFNL

>Ciona savignyi RNF225
MASASKSFKNKLEDDLLTCPICCSLYSDPRVLKCQHSFCRSCLERSVKASRTNCNSINCA
VCRKTTPLGRKRLQELPKNYVIIGLSQMVKDHKGNGGSSVDAEEPSMKENVVDVQQLVKV
FNKKSISSKKKSGKDKNDNKLIPNVMDGGDDSSDFSTSASDFSSEFTPTDSDQSGSSGSS
SGSSGSSGSSSASTESSSEDCDDSPEVLPPTPTMKSSLGIKIGMKVYVFSSRGKPHPGVV
RVLEKFKRKPPGQTRVYAGVQLEKENTRLTTDDIEDMDDSYLFNWLMDFDEPCTVVRVDQ
CCPAKIIESVMK

>Branchiostoma floridae RNF225
MATGGESPQGSLQIENFECSICLQVYMRPKVLSCGHTFCKECLVPLAKGSKSFKCPTCQATIRLERAGRA
GIEGLIDNISLSNLRDYVETSNRQGPRGSENPTVVSDESQCPKHHGEELRYYCPPCDAAICEECMFEDHN
THGVTRLRRTLQEQSIKAKEVCEEGTRLIETIRHKITELEQSRKRLAVEEGTLVKAVDQTARKLADDFAT
EVTKRANSLKDELHNLFAPSNSLVLDCKESLEIRLAQLVSSVESLEKSINQGEAIVVFSGRKMLKDLIEQ
SSQPTKAEQDVETGNIAVFTPTPYSFPAITLGKVQATSKIWERKKETDEEKVETSAAPKEDSPAPEVDGV
TNNNEAEENDEDIVEIEDKSGLKYKCREDQRNPLNSEGKKQYDRSFLLQFQPECIDKPDGLPKIVDVVLD
KPLIITPQQMQGRPQDFRGMGGSPGIPDPWPIPSYMKSPGGDHRRMEVFEKMQGILDKLTSQNFQTLCQQ
VLDLDIVTEERLKGVIDLVFEKAITEPNFSVAYANMCRCLIHLKVPMKDNPKQTVNFRKLLLNRCQGEFE
RDIDNKMEKDERMRKIELAKNEDEKKKMQAELEEEMANASRRSLGNIRFIGELFKLKILAENIMHDCVVK
LIKFNDEESLECLCRLLTMIGKDLDVERAKPLMDQYFEQLERNTKQKKTSSRLRFMIQDVLELRRVGIPQ
SLK

>Strongylocentrotus purpuratus RNF225
MADALKNAIAQSLECPVCLNTFTDPKILSCSHTYCKTCLDNLLDCHGNDQMLRCPVCRAETQVPNQEVSK
LPTNLALKSLIEDVKNHYQFCTNCKSEDKPQAVVYCQDCGKYFCSTCHNKHSQWPDFISHDVLAMSEIVS
GKVSVRRYRKCRKHPKDDEEWFCSDCRRFACFKCVVMEHTNEGHRVIEAAVYESNHMKSIEDLKSKADKK
RSCFQKYIDFIDEQKEHVSNVQKQCTDDINKAYEESVRQLTKKKEVLIGEVKGRTEGVEKELDEIKKSAQ
EHITHFTTIADVVTNRTKIPLDTDALAAHDTLCQDLQEALKQEDPDYKQSIQLSRKGKSVSFKRNVGMDE
LALGKIANAFAFKNIALPTKNSMNIMVGTPDGRMAVGCDTGGVEIFSADGQLQQTVLKGVKIRGVGFLSD
DRYVILDCSNNISLYRLQYTKLNVVFETLYNDEGEWAILNVGGDDQIYVSYWKAQKIQVFSPAGGKAIRE
IPCDGYEPQQITSYNDYLVTRSWDAIRLIDKEGVVKYELRELGYFPYATVSQGNTILIAKVNHDVGLVSI
DEYTNELKHIQNLVFDYKIEKPERWWYYLQQYRSGEIAFCTPDRLYIFHWK

>Anopheles gambiae RNF225
MSTNGWIHCNICFHSFERKERKFYHLSCRHVLCKLCMAKTNRGTMCPLCKKPLERFTELNNQMERKEKMY
YDPGSLKVFGVAYQSVIFQHKQREDMIRGILRCRSAVTQMKEMENAMRQKIVETQRRYEKFRTHRRNLQE
GLRQISPRYGSGPTVVQGAGCAGRTPLQPLTGNRASPQLALANRDGDPASRRYQDKGRPETVTFAASPES
SSADFSFGTPAPSNRSATVTDGSFTPAEQQHARLSTPQVSTERFFREGNKPAVTGQGDGVFADDSGISSM
QTPTSSLSFTGSSRMAQGMQQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQLKQQQQQHQQLK
QQRQHPYTPSTPARHLPGTSSYRHSQIFPPTVPRP

>Caenorhabditis elegans RNF225
MSEKNTENPEILSTVLAGYCQCQVCYQPFNETTKLARSLHCGHTFCTECIRNVQNYGNSP
HLECPTCRAETKSNIENVAPNFSIMELARNFGLLGPPVDAPRTSSSCCSNKTNRDEMIDN
KFRELLVEVKSSLIHHFDVFRIKSQLWSLAEAEREVSNMAEALAGAVSECYYDSDDNSDA
NSEDQRVRTMLRFLAARDSDSETTEDSTDFLESNTTVPDRTLGGGFYRNFRRLFGDENEL
DDPGVVSGIEEAAAYRRFRGLFGNDNDSISQVSTTSGSFGNHENDHFCPFSDDDNISECS
DDYYPTLVMPETDEEETYKSFRCLFGDENGNLDFSDDEKEFSDNESSLSSVSTEDFPNSH
IEEAGRVLHRFLLLSALLNEE

>Nematostella vectensis RNF225
MATVRDEGYSPQSLNCRACHKVFTEPKILDCLHTFCQKCLGTHDILGAGTNSIVCPLCRKPTPIPESGVE
SLPSNFLLNNALDQLSVKSSKEYILHCTNCEDASDATQTLHRPSFCMQHKDEMFMFYCLECEGLICRECT
ILDHRGHKYAGLKDALEGRKILIDDLLGFCTVKKIPPVQDAVYEVKAMTAKLRARADAVKNDISSAADEC
VKRILARKQELLTQVDNMTQSKEGVLQAQQEQLEMELFRYTSSRDFVENVLLHGNEVEIIQLRQLMTNRL
NELNGVKLDYKEPEENDVIDYILDSEAVQKVAMTLGHVTTSQTFASLCIAKGLGVMSGKVGVESFFTIET
KDRFGNTTFEGDHCTPIQVIIQAPEGFFLGNHVANKRDGTLLVRYTPVTRGKHVIVVSIRGRQIEGSPFT
VKVVEGIDYKNVMAVFMKIGSQGTKSGEFRQPSSVATDTDGNIYVTDFVNCRVQKFDMFGKHLKDIGTKG
SKDGQLMNPCGVVVGSDGTLIVSDWDNHRVQMFSPEGKFVSKIGRKGSENGNFLHPSGLALNQDGDLVVI
DKDNNRVQVLKLDGSHVMSFGSLGNADGQLDCPSHVAVTPDNGYLVTDTGNNRIVKFDASGNHVMSFGTK
GSGEGRLDRPAGIAIDPEGFIIISDFQNHRVQVHSPEGEFYATFGSEGNIEGQFKFPSGIALTLDGHVIV
ADRHNHRIQVF

>Hydra magnipapillata RNF225
MAALSSSQVLENIHEQLVCAICLERFDDPKLLNCLHSFCCQCLHKIINQKPSDVIECPTCREVTKIPING
LKELKTNFFINSLLDLVHHKIEELKNEKKKGEQCEQCQEHDNATSRCIECDLVLCGGCIADHRRNRSTLD
HRIVVMEDDEDVNKQSLDKYTLTFCRYHTRNVIKYFCNTCDEAVCRVCTILEHREHQFVYPKDAIPQQKP
IIEELLEKTKENIPLLKATIKDIHSMSNTLRECRESLLLEINAKTQARIEALLLAKAKLLEELDRIHRGK
QKTLSLQSDAVELELGKISGCCAFAENVLKFGNEVEVLQIKSRLQQLNSITIKLDPEEDDSIQYVSESES
IEAVMESLGKICSSQTFASLSYAEGEGLETPRVGIESNIIVHAQNRHGKKKSGSDRVEAVMVLPDETKRT
LNALDNDDGTYTIRFTPDINGRHLLHITIKTKPINGSPFEIFVINRREYSEVGPSLLKFGQYGSKRREFK
SPFGVASDNEGFIYVADSYNHRIQTFGPRGEFVHMFGSHGDRKGEFNCPTDVAVSPNSKLVVCDNGNNRI
QVFGRNGNFIAKFGREGIKNGNFKSPWGVTVSPVSREIIVADTDNHRIQIFSSDGKFIMKFGCNGDRPGQ
FNSPCYVAANAENEHILVSDSKNHRIQIFDRRGKFIQLFGTQGQEDGQFNHPRGLSMDIANNIIVSDMGN
HRLQILTYAGKMVKCVGTEGNGDGQLSFPESVAVTPNGFIIVSDLSNNRIQVF

>Amphimedon queenslandica RNF225
MTLEEAPERDLFEELEENALTCAVCLGVYEDPRALDCHHVFCRSCLEHLPLVNQDGVNNLGCPTCRHLVP
LPPNGVAGFRVSFQTNNLKAFVEANRDRAVPVAVPRKQTCGSCTSENVSGFCTECRSTFCDGCTDLHLPT
CTKEATALNGASGTDKTNGHHQAPMPLDPAPLMQSDDWNVNTNTCVIHRQIADMFCENCEETICSRCVKR
FPHTEHESSCIPLAEALVRYRELLEDRLNPINKEIDFISTQLKPFSHRESEINEQGDTVKAQIHSRAEYL
IALIIESEEELSVKVDKVIKSKLHSLDERRESIEVTQEELKNLKDRIELCLKTGNQEDLLKTKQWIPKIN
HVMFNIDATKLKPSEVADLSFVPDEVSDSLTRLGNLSSTFEGKDPSCDVQILEQHLFIDKSPDVTLVLSV
KYTNLPFVIIPLSSMKCIIKSSNRSFIKAIISLGDAAHQYVVKFRPTSNGLYKLSLKIDDIETEDQAVLI
PFNPMYLPNITPTTVMKYIRKPIGLAVAQDKRIIVLNDFNEVVILDMHGRNIRTLSRPNNGSRFTSTRHA
AVTSDGCFLMTDQYSILKVTMDGQLLKTIGSKGNGRLQFNTPFGITIDEKTGKVYVADLLNHRIQVLNRD
YSFSHSFGSRGKALGQFEQPNGTALDSQGNVFVSDTFNHRIQKFSQSGEYISYFGSEGPNPGQLKLPEKI
VINNDYIYVTEHLNARVSVFTTEGNFVRCFGKQSIEGGLVWPRGLTFDEDGSLYVCDFRSHCIFKY